The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Human Ccr4–Not complexes contain variable deadenylase subunits
Open accessAbstract
The Ccr4–Not complex is evolutionarily conserved and important for regulation of mRNA synthesis and decay. The composition of the yeast complex has been well described. Orthologues of the yeast Ccr4–Not components have been identified in human cells including multiple subunits with mRNA deadenylase activity. In the present study, we examine the composition of the human Ccr4–Not complex in an in-depth proteomic approach using stable cell lines expressing tagged CNOT proteins. We find at least four different variants of the human complex, consisting of seven stable core proteins and mutually exclusive associated mRNA deadenylase subunits. Interestingly, human CNOT4 is in a separate ~200 kDa complex. Furthermore, analyses of associated proteins indicate involvement of Ccr4–Not complexes in splicing, transport and localization of RNA molecules. Taken together, human Ccr4–Not complexes are heterogeneous in composition owing to differences in their deadenylase subunits, which may reflect the multi-functionality of these complexes in cellular processes.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Mousson, F., Kolkman, A., Pijnappel, W. W. M. P., Timmers, H. T. M., & Heck, A. J. R. (2008) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m700306-mcp200 · Cited by 67
Self-citation
Cited by
GW182 Proteins Directly Recruit Cytoplasmic Deadenylase Complexes to miRNA Targets
Braun, J. E., Huntzinger, E., Fauser, M., & Izaurralde, E. (2011) · Molecular Cell · doi:10.1016/j.molcel.2011.09.007 · Cited by 377
Fabian, M. R., Cieplak, M. K., Frank, F., Morita, M., Green, J., Srikumar, T., Nagar, B., Yamamoto, T., Raught, B., Duchaine, T. F., et al. (2011) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2149 · Cited by 329
Human Pumilio Proteins Recruit Multiple Deadenylases to Efficiently Repress Messenger RNAs
Van Etten, J., Schagat, T. L., Hrit, J., Weidmann, C. A., Brumbaugh, J., Coon, J. J., & Goldstrohm, A. C. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.373522 · Cited by 205
Sandler, H., Kreth, J., Timmers, H. T. M., & Stoecklin, G. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr011 · Cited by 221
Self-citationMarchese, F. P., Aubareda, A., Tudor, C., Saklatvala, J., Clark, A. R., & Dean, J. L. E. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.136473 · Cited by 136
Metadata
- Authors
- Lau, N.-C., Kolkman, A., van Schaik, F. M. A., Mulder, K. W., Pijnappel, W. W. M. P., Heck, A. J. R., & Timmers, H. T. M.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 2009-08-27
- Volume
- 422
- Issue
- 3
- Pages
- 443-453
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W2169095006
- Cited by
- 202
- References
- 1
- FWCI
- 4.32
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Human Ccr4–Not complexes contain variable deadenylase subunits. https://app.cochat.ai/papers/10.1042/bj20090500
- The paper
- Lau, N.-C., Kolkman, A., van Schaik, F. M. A., Mulder, K. W., Pijnappel, W. W. M. P., Heck, A. J. R., & Timmers, H. T. M. (2009). Human Ccr4–Not complexes contain variable deadenylase subunits. Biochemical Journal. https://doi.org/10.1042/bj20090500
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
