The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cholesterol-dependent phase separation in cell-derived giant plasma-membrane vesicles
Open accessAbstract
Cell-derived GPMVs (giant plasma-membrane vesicles) enable investigation of lipid phase separation in a system with appropriate biological complexity under physiological conditions, and in the present study were used to investigate the cholesterol-dependence of domain formation and stability. The cholesterol level is directly related to the abundance of the liquid-ordered phase fraction, which is the majority phase in vesicles from untreated cells. Miscibility transition temperature depends on cholesterol and correlates strongly with the presence of detergent-insoluble membrane in cell lysates. Fluorescence correlation spectroscopy reveals two distinct diffusing populations in phase-separated cell membrane-derived vesicles whose diffusivities correspond well to diffusivities in both model systems and live cells. The results of the present study extend previous observations in purified lipid systems to the complex environment of the plasma membrane and provide insight into the effect of cholesterol on lipid phase separation and abundance.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Cited by
The mystery of membrane organization: composition, regulation and roles of lipid rafts
Sezgin, E., Levental, I., Mayor, S., & Eggeling, C. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.16 · Cited by 2,124
Self-citationPalmitoylation regulates raft affinity for the majority of integral raft proteins
Levental, I., Lingwood, D., Grzybek, M., Coskun, Ü., & Simons, K. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1016184107 · Cited by 549
Self-citationElucidating membrane structure and protein behavior using giant plasma membrane vesicles
Sezgin, E., Kaiser, H.-J., Baumgart, T., Schwille, P., Simons, K., & Levental, I. (2012) · Nature Protocols · doi:10.1038/nprot.2012.059 · Cited by 527
Self-citationGreasing Their Way: Lipid Modifications Determine Protein Association with Membrane Rafts
Levental, I., Grzybek, M., & Simons, K. (2010) · Biochemistry · doi:10.1021/bi100882y · Cited by 420
Self-citationSezgin, E., Levental, I., Grzybek, M., Schwarzmann, G., Mueller, V., Honigmann, A., Belov, V. N., Eggeling, C., Coskun, Ü., Simons, K., et al. (2012) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2012.03.007 · Cited by 334
Self-citation
Metadata
- Authors
- Levental, I., Byfield, F. J., Chowdhury, P., Gai, F., Baumgart, T., & Janmey, P. A.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 2009-11-11
- Volume
- 424
- Issue
- 2
- Pages
- 163-167
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W2021867116
- Cited by
- 162
- References
- 1
- FWCI
- 3.00
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Cholesterol-dependent phase separation in cell-derived giant plasma-membrane vesicles. https://app.cochat.ai/papers/10.1042/bj20091283
- The paper
- Levental, I., Byfield, F. J., Chowdhury, P., Gai, F., Baumgart, T., & Janmey, P. A. (2009). Cholesterol-dependent phase separation in cell-derived giant plasma-membrane vesicles. Biochemical Journal. https://doi.org/10.1042/bj20091283
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
