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Cited by 900

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Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts. The effect of hydrogen peroxide and of Paraquat

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Law, M Y, Charles, S A, Halliwell, B·1983·Biochemical Journal

Abstract

The stroma of spinach chloroplasts contains ascorbic acid and glutathione at millimolar concentrations. [Reduced glutathione]/[oxidized glutathione] and [ascorbate]/[dehydroascorbate] ratios are high under both light and dark conditions and no evidence for a role of oxidized glutathione or dehydroascorbate in the dark-deactivation of fructose bisphosphatase could be obtained. Addition of H2O2 to chloroplasts in the dark decreases the above ratios, an effect that is reversed on illumination. Addition of Paraquat to illuminated chloroplasts caused a rapid oxidation of reduced glutathione and ascorbate, and apparent loss of dehydroascorbate. Paraquat rapidly inactivated fructose bisphosphatase activity, as assayed under physiological conditions.

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Metadata

Authors
Law, M. Y., Charles, S. A., & Halliwell, B.
Venue
Biochemical Journal
Published
1983-03-15
Volume
210
Issue
3
Pages
899-903
ISSN
0264-6021 (ISSN-L)
OpenAlex
W1898874031
Cited by
900
References
0
FWCI
2.45
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts. The effect of hydrogen peroxide and of Paraquat. https://app.cochat.ai/papers/10.1042/bj2100899
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Law, M. Y., Charles, S. A., & Halliwell, B. (1983). Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts. The effect of hydrogen peroxide and of Paraquat. Biochemical Journal. https://doi.org/10.1042/bj2100899

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.