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High resolution 1H n.m.r. studies of vertebrate blood and plasma
Open accessAbstract
Spin echo Fourier transform proton n.m.r. spectra of whole blood contain resonances from both erythrocytes and plasma. A large number of well-resolved signals from mobile protons of low-molecular-weight metabolites in plasma and serum have been identified. Spectra from the plasmas of eight animal species and commercial, quality control sera are compared. CaEDTA2- and MgEDTA2- resonances can be used for the simultaneous determination of EDTA-chelatable calcium and magnesium concentrations in intact plasma and other biological fluids. Cholesterol is too immobile to contribute to the spectra of intact plasma, but is readily estimated by n.m.r. in both its free and esterified forms after extraction into methanol.
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Cited by
Beckonert, O., Keun, H. C., Ebbels, T. M. D., Bundy, J., Holmes, E., Lindon, J. C., & Nicholson, J. K. (2007) · Nature Protocols · doi:10.1038/nprot.2007.376 · Cited by 2,109
Self-citationMetabonomics: a platform for studying drug toxicity and gene function
Nicholson, J. K., Connelly, J., Lindon, J. C., & Holmes, E. (2002) · Nature Reviews Drug Discovery · doi:10.1038/nrd728 · Cited by 1,914
Self-citationDona, A. C., Jiménez, B., Schäfer, H., Humpfer, E., Spraul, M., Lewis, M. R., Pearce, J. T. M., Holmes, E., Lindon, J. C., & Nicholson, J. K. (2014) · Analytical Chemistry · doi:10.1021/ac5025039 · Cited by 540
Self-citationNMR‐based metabonomic approaches for evaluating physiological influences on biofluid composition
Bollard, M. E., Stanley, E. G., Lindon, J. C., Nicholson, J. K., & Holmes, E. (2005) · NMR in Biomedicine · doi:10.1002/nbm.935 · Cited by 473
Self-citationLindon, J. C., Nicholson, J. K., & Everett, J. R. (1999) · Annual Reports on NMR Spectroscopy · doi:10.1016/s0066-4103(08)60035-6 · Cited by 395
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Metadata
- Authors
- Nicholson, J. K., Buckingham, M. J., & Sadler, P. J.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1983-06-01
- Volume
- 211
- Issue
- 3
- Pages
- 605-615
- ISSN
- 0264-6021 (ISSN-L)
- OpenAlex
- W1747625137
- Cited by
- 255
- References
- 0
- FWCI
- 2.61
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Nicholson, J. K., Buckingham, M. J., & Sadler, P. J. (1983). High resolution 1H n.m.r. studies of vertebrate blood and plasma. Biochemical Journal. https://doi.org/10.1042/bj2110605
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