The DOI matches its Crossref record; 25 of the 29 references it cites were found in a registry or an index.
Design and synthesis of a consensus signal sequence that inhibits protein translocation into rough microsomal vesicles.
Open accessAbstract
Most signal sequences are found to vary considerably in length and primary sequence, but possess some common structural features. Analysis of known signal sequences has led to the design of a 19-residue sequence that, although not a naturally occurring signal, possesses the structural features that commonly occur in pre-proteins. This peptide has been synthesized by solid-phase methods, and has been shown to inhibit, in a concentration-dependent manner, the processing in vitro of nascent pre-prolactin, pre-forms of pancreatic digestive enzymes, and pre-placental lactogen. The peptide acts at the cytoplasmic surface of microsomal vesicles added to the protein translation system, preventing translocation of the nascent chains to the lumenal space of vesicles where signal peptidase normally cleaves to remove the signal from nascent pre-proteins.
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“The first characterized inhibitors of the Sec complex were synthetic signal peptides that have been shown to inhibit the eukaryotic Sec61 complex (Austen et al., 1984).”
From The Dynamic SecYEG Translocon (2021) · cites this paper as Austen et al., 1984
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Cites
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
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Research works printed but not linked: 9
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Cited by
A conformational preference parameter to predict helices in integral membrane proteins
Rao, J., & Argos, P. (1986) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/0167-4838(86)90295-5 · Cited by 547
Roise, D., & Schatz, G. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)68809-x · Cited by 391
ERO1-L, a Human Protein That Favors Disulfide Bond Formation in the Endoplasmic Reticulum
Cabibbo, A., Pagani, M., Fabbri, M., Rocchi, M., Farmery, M. R., Bulleid, N. J., & Sitia, R. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.7.4827 · Cited by 298
Molecular Mechanisms of Protein Secretion: The Role of the Signal Sequence
Briggs, M. S., & Gierasch, L. M. (1986) · Advances in protein chemistry · doi:10.1016/s0065-3233(08)60527-6 · Cited by 221
Calcitonin gene-related peptide stimulates cyclic AMP formation in rat aortic smooth muscle cells
Kubota, M., Moseley, J. M., Butera, L., Dusting, G. J., Macdonald, P. S., & Martin, T. J. (1985) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(85)90992-1 · Cited by 196
Metadata
- Authors
- Austen, B. M., Hermon-Taylor, J., Kaderbhai, M. A., & Ridd, D. H.
- Affiliations
- St George's, University of London (GB)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1984-11-15
- Volume
- 224
- Issue
- 1
- Pages
- 317-325
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1144429
- OpenAlex
- W2129132769
- Cited by
- 53
- References
- 29
- Citations by year
- 2021: 1 · 2015: 1
- FWCI
- 2.43
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Physical Sciences › Chemistry › Organic Chemistry › Chemical Synthesis and Analysis
- Keywords
- signal peptide, protein translocation, signal peptidase, microsomal vesicles, prolactin, pancreatic enzymes, placental lactogen, signal sequence cleavage
- MeSH terms
- Amino Acid Sequence, Animals, Cattle, Dogs, Electrophoresis, Polyacrylamide Gel, Microsomes, Models, Molecular, Peptides, Placental Lactogen, Prolactin, Protein Precursors, Proteins, Protein Sorting Signals, In Vitro Techniques
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- CoChat. (2026). Citation check: Design and synthesis of a consensus signal sequence that inhibits protein translocation into rough microsomal vesicles. https://app.cochat.ai/papers/10.1042/bj2240317
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- Austen, B. M., Hermon-Taylor, J., Kaderbhai, M. A., & Ridd, D. H. (1984). Design and synthesis of a consensus signal sequence that inhibits protein translocation into rough microsomal vesicles. Biochemical Journal. https://doi.org/10.1042/bj2240317
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
