The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
The effect of the inhibitor diphenylene iodonium on the superoxide-generating system of neutrophils. Specific labelling of a component polypeptide of the oxidase
Open accessAbstract
NADPH-dependent superoxide production by the solubilized oxidase of neutrophils was inhibited 36% by diphenylene iodonium at a 1:1 stoichiometry with the enzyme flavoprotein content. Addition of diphenylene iodonium strongly inhibited the NADPH-dependent reduction of both FAD and cytochrome b-245 in steady-state kinetic experiments. Incubation of solubilized enzyme with diphenylene [125I]iodonium resulted in the specific labelling of a polypeptide of Mr 45,000. In the presence of NADPH the amount of label incorporated into the polypeptide was reduced. There was no difference in labelling between enzyme prepared from stimulated or unstimulated cells.
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“Diphenylene iodonium (DPI) (FIGURE 10) is a nonspecific inhibitor of all NOX isoforms (18, 197, 246).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [197]
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Cites
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Research works printed but not linked: 1
- Bloxham, D. P. (1979) Biochem. Soc. Trans. 7, 103-106
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Nitric oxide synthases in mammals
Knowles, R. G., & Moncada, S. (1994) · Biochemical Journal · doi:10.1042/bj2980249 · Cited by 2,752
Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps
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Generation of reactive oxygen species by the mitochondrial electron transport chain
Liu, Y., Fiskum, G., & Schubert, D. (2002) · Journal of Neurochemistry · doi:10.1046/j.0022-3042.2002.00744.x · Cited by 1,260
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Oxidants and human disease: some new concepts 1
Halliwell, B. B. (1987) · The FASEB Journal · doi:10.1096/fasebj.1.5.2824268 · Cited by 1,205
Metadata
- Authors
- Cross, A. R., & Jones, O. T. G.
- Affiliations
- University of Bristol (GB)
- ORCID
- Jones, O T G
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1986-07-01
- Volume
- 237
- Issue
- 1
- Pages
- 111-116
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1146954
- OpenAlex
- W1801129364
- Cited by
- 551
- References
- 22
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 1 · 2023: 5 · 2022: 7 · 2021: 5 · 2020: 9 · 2019: 12 · 2018: 13 · 2017: 14 · 2016: 9 · 2015: 13 · 2014: 9 · 2013: 19 · 2012: 20
- FWCI
- 5.47
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Medical Research Council
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Bioactive Compounds and Antitumor Agents
- Keywords
- diphenyleneiodonium, neutrophil NADPH oxidase, superoxide production, flavoprotein, enzyme inhibition, FAD reduction, NADPH-dependent reduction, respiratory burst
- MeSH terms
- Animals, Electrophoresis, Polyacrylamide Gel, NADH, NADPH Oxidoreductases, NADP, Neutrophils, Onium Compounds, Protein Binding, Swine, NADPH Oxidases, In Vitro Techniques
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