Identification of specific binding sites for keratan sulphate proteoglycans and chondroitin-dermatan sulphate proteoglycans on collagen fibrils in cornea by the use of cupromeronic blue in ‘critical-electrolyte-concentration’ techniques
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Proteoglycans (PGs) in bovine corneal stroma were stained with Cupromeronic Blue in ‘critical-electrolyte-concentration’ (CEC) methods for electron microscopy, and were located vis-à-vis collagen fibril a-e banding patterns. Keratanase and chondroitin ABC lyase digestion showed that a + c-band- and d + e-band-associated PGs were keratan sulphate-rich and chondroitin (dermatan) sulphate-rich respectively. The CEC pattern proved that the keratan sulphate PGs at the a and c bands differed. Comparison of their CECs with their behaviour on anion-exchange chromatography confirmed previous (indirect) attempts at identification [Scott & Haigh (1985) Biosci. Rep. 5, 765-774]. Similar arguments were applied to the dermatan sulphate PGs at the d and e bands. These results strongly support the one-PG-one-binding-site hypothesis [e.g. Scott (1988) Biochem. J. 252, 313-323]. Remarkable inter-species variations in the keratan sulphate PG patterns contrast with the relatively constant picture of dermatan sulphate PG-collagen fibril interactions.
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The individual proteoglycan core proteins are thought to bind to collagen fibrils at specific axial sites along the collagen fibrils.
“The individual proteoglycan core proteins are thought to bind to collagen fibrils at specific axial sites along the collagen fibrils (Meek et al., 1986, Scott and Haigh, 1988).”
From Corneal structure and transparency (2015) · cites this paper as Scott and Haigh, 1988
The surface gaps are a primary binding site for dermatan sulphate-containing proteoglycans, which are necessary for preventing adjacent fibrils from fusing together.
“They are also a primary binding site for dermatan sulphate-containing proteoglycans (Scott and Haigh, 1988), which, as we will also see later, are necessary for preventing adjacent fibrils from fusing together (Chen and Birk, 2013).”
From Corneal structure and transparency (2015) · cites this paper as Scott and Haigh, 1988
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Research works printed but not linked: 4
- Scott, J. E. (1975) Philos. Trans. R. Soc. London B 271, 235-242
- Evered, D. & Whelan, J. (eds.) (1986) Ciba Found. Symp. 124
- Scott, J. E. (1973) Biochem. Soc. Trans. 1, 787-806
- Scott, J. E. & Hughes, E. W. (1982) J. Microscop. (Oxford) 129, 209-219
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Corneal structure and transparency
Meek, K. M., & Knupp, C. (2015) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2015.07.001 · Cited by 853 · see citing works
- Authors
- Scott, J. E., & Haigh, M.
- Affiliations
- University of Manchester (GB)
- ORCID
- Scott, J E
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1988-07-15
- Volume
- 253
- Issue
- 2
- Pages
- 607-610
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1149341
- OpenAlex
- W2432902234
- Cited by
- 140
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 5 · 2014: 2 · 2013: 2 · 2012: 3
- FWCI
- 2.85
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal surgery and disorders
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal Surgery and Treatments
- Keywords
- chondroitin sulfate proteoglycans, corneal stroma, collagen fibrils, interspecific variation
- MeSH terms
- Lumican, Animals, Binding Sites, Cattle, Chondroitin, Collagen, Cornea, Dermatan Sulfate, Coloring Agents, Electrolytes, Glycosaminoglycans, Indicators and Reagents, Indoles, Keratan Sulfate, Microscopy, Electron, Organometallic Compounds, Chondroitin Sulfate Proteoglycans, Proteoglycans, Rabbits
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