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Cited by 63Retinoids in leukemia and cellular processesGenomics and Chromatin DynamicsCytokine Signaling Pathways and Interactions

Differences in expression of transcription factor AP-1 in human promyelocytic HL-60 cells during differentiation towards macrophages versus granulocytes

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Mollinedo, F, Gajate, C, Tugores, A, Flores, I, Naranjo, J R·1993·Biochemical Journal
DOI 10.1042/bj2940137PMC PMC1134576
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Abstract

Commitment of HL-60 cells to macrophage or granulocytic differentiation was achieved by incubation with 4 beta-phorbol 12-myristate 13-acetate (PMA) for 30-60 min or with dimethyl sulphoxide (DMSO) for 24 h respectively. The commitment stage towards PMA-induced macrophage differentiation was associated with increases in jun B and c-fos mRNA levels, as well as with an increase in the binding activity of transcription factor AP-1. Nevertheless, gel retardation analysis indicated that the AP-1 activity detected in untreated cells was drastically reduced during the commitment stage of DMSO-induced HL-60 differentiation towards granulocytes. When HL-60 cells were treated with sodium butyrate, which induced monocytic differentiation, a remarkable increase in AP-1 binding activity was detected. Treatment of HL-60 cells with 1 alpha,25-dihydroxyvitamin D3, another monocytic differentiation agent, induced a weak, but appreciable, increase in AP-1 activity. Furthermore, addition of sodium butyrate or 1 alpha,25-dihydroxyvitamin D3 to HL-60 cells induced the expression of c-fos, c-jun, jun B and jun D proto-oncogenes. In contrast, when HL-60 cells were treated with retinoic acid, a granulocytic differentiation inducer, no enhanced AP-1 binding activity was observed, and only a weak increase in jun D mRNA level was detected. These data indicate that formation of AP-1 is not required for the induction of HL-60 differentiation towards granulocytes, whereas induction of monocytic differentiation is correlated with an increase in AP-1 activity. The differential expression of AP-1 activity may be critical in the differentiation of HL-60 cells towards monocytic or granulocytic lineages.

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Authors
Mollinedo, F., Gajate, C., Tugores, A., Flores, I., & Naranjo, J. R.
Affiliations
  • Consejo Superior de Investigaciones Científicas (ES)
  • Hospital Universitario de La Princesa (ES)
ORCID
Venue
Biochemical Journal
Publisher
Portland Press Ltd.
Published
1993-08-15
Volume
294
Issue
1
Pages
137-144
ISSN
  • 0264-6021 (ISSN-L)
  • 1470-8728
DOI
10.1042/bj2940137
PMC
PMC1134576
OpenAlex
W2401320033
Cited by
63
References
55
Citations by year
2020: 1 · 2019: 1 · 2012: 1
FWCI
1.42
Type
journal article
Language
en
Open access
Locations
  • Biochemical Journal (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Acceda (Universidad de Las Palmas de Gran Canaria) (submittedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
Keywords
AP-1 transcription factor, HL-60 cells, promyelocytic leukemia cells, macrophage differentiation, granulocytic differentiation, monocyte differentiation, JunB, JunD, c-fos, c-Jun, sodium butyrate, retinoic acid, phorbol myristate acetate, dimethyl sulfoxide, lineage commitment, proto-oncogene expression
MeSH terms
Base Sequence, Binding Sites, Butyrates, Calcitriol, Cell Differentiation, Cell Line, Dimethyl Sulfoxide, Granulocytes, Humans, Macrophages, Molecular Sequence Data, Oligodeoxyribonucleotides, RNA, Messenger, Tetradecanoylphorbol Acetate, Tretinoin, Tumor Cells, Cultured, Proto-Oncogene Proteins c-jun, Genes, jun, Genes, fos, Butyric Acid

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