The DOI matches its OpenAlex record; 32 of the 32 references it cites were found in a registry or an index.
Membrane-anchored metalloprotease MDC9 has an α-secretase activity responsible for processing the amyloid precursor protein
Open accessAbstract
MDC9, also known as meltrin gamma, is a membrane-anchored metalloprotease. MDC9 contains several distinct protein domains: a signal sequence followed by a prodomain and a domain showing sequence similarity to snake venom metalloproteases, a disintegrin-like domain, a cysteine-rich region, an epidermal-growth-factor-like repeat, a transmembrane domain and a cytoplasmic domain. Here we demonstrate that MDC9 expressed in COS cells is cleaved between the prodomain and the metalloprotease domain. Further, when MDC9 was co-expressed in COS cells with amyloid precursor protein (APP695) and treated with phorbol ester, APP695 was digested exclusively at the alpha-secretory site in MDC9-expressing cells. When an artificial alpha-secretory site mutant was also co-expressed with MDC9 and treated with phorbol ester, APP secreted by alpha-secretase was not increased in conditional medium. Inhibition of MDC9 by a hydroxamate-based metalloprotease inhibitor, SI-27, enhanced beta-secretase cleavage. These results suggest that MDC9 has an alpha-secretase-like activity and is activated by phorbol ester.
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Cites
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Low cholesterol stimulates the nonamyloidogenic pathway by its effect on the α-secretase ADAM 10
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Metadata
- Authors
- KOIKE, H., Tomioka, S., Sorimachi, H., Saido, T. C., Maruyama, K., Okuyama, A., Fujisawa‐Sehara, A., Ohno, S., Suzuki, K., & Ishiura, S.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1999-10-08
- Volume
- 343
- Issue
- 2
- Pages
- 371-375
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W2029391653
- Cited by
- 278
- References
- 32
- FWCI
- 9.86
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Membrane-anchored metalloprotease MDC9 has an α-secretase activity responsible for processing the amyloid precursor protein. https://app.cochat.ai/papers/10.1042/0264-6021:3430371
- The paper
- KOIKE, H., Tomioka, S., Sorimachi, H., Saido, T. C., Maruyama, K., Okuyama, A., Fujisawa‐Sehara, A., Ohno, S., Suzuki, K., & Ishiura, S. (1999). Membrane-anchored metalloprotease MDC9 has an α-secretase activity responsible for processing the amyloid precursor protein. Biochemical Journal. https://doi.org/10.1042/0264-6021%3A3430371
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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