The DOI matches its Crossref record; 158 of the 158 references it cites were found in a registry or an index.
Multicellular spheroids: a three‐dimensional in vitro culture system to study tumour biology
Open accessAbstract
The growth of tumour cells as three‐dimensional multicellular spheroids in vitro has led to important insights in tumour biology, since properties of the in vivo‐tumour such as proliferation or nutrient gradients, can be studied under controlled conditions. While this review starts with an update of recent data on spheroid monocultures, especially concerning tumour microenvironment and therapeutic modalities, the main emphasis is put on the spectrum of heterologous cultures which have evolved in previous years. This type of culture includes tumour cell interaction with endothelial, fibroblast or immunocompetent cells. The relation of the spheroid culture model to other types of three‐dimensional culture and our critical evaluation and presentation of the technical aspects of growing and analysing spheroids are included in the text. These topics are chosed to help the experimental pathologist design experiments with tumour spheroids and to stimulate discussion.
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Cites
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Cited by
Spheroid-based drug screen: Considerations and practical approach
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Self-citationMulticellular tumor spheroids: An underestimated tool is catching up again
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Self-citationOpportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy
Mehta, G., Hsiao, A. Y., Ingram, M., Luker, G. D., & Takayama, S. (2012) · Journal of Controlled Release · doi:10.1016/j.jconrel.2012.04.045 · Cited by 1,172
Kelm, J. M., Timmins, N. E., Brown, C. J., Fussenegger, M., & Nielsen, L. K. (2003) · Biotechnology and Bioengineering · doi:10.1002/bit.10655 · Cited by 934
Three-dimensional culture systems in cancer research: Focus on tumor spheroid model
Nath, S., & Devi, G. R. (2016) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2016.03.013 · Cited by 919
Metadata
- Authors
- KUNZ‐SCHUGHART, L. A., KREUTZ, M., & KNUECHEL, R.
- Venue
- International Journal of Experimental Pathology
- Publisher
- Wiley
- Published
- 1998-02
- Volume
- 79
- Issue
- 1
- Pages
- 1-23
- ISSN
- 0959-9673 (ISSN-L)
- 1365-2613
- OpenAlex
- W1527382852
- Cited by
- 324
- References
- 158
- FWCI
- 3.23
- Type
- journal article
- Language
- en
- Open access
- green
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- KUNZ‐SCHUGHART, L. A., KREUTZ, M., & KNUECHEL, R. (1998). Multicellular spheroids: a three‐dimensional in vitro culture system to study tumour biology. International Journal of Experimental Pathology. https://doi.org/10.1046/j.1365-2613.1998.00051.x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
