The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Fluorometric assessment of Gram-negative bacterial permeabilization
Abstract
Uptake of the fluorescent probe 1-N-phenylnaphthylamine (NPN), as adapted to an automated spectrofluorometer enabling multiwell reading of microtitre plates, was applied to determine permeability changes in Gram-negative bacteria. An intact outer membrane is a permeability barrier, and excludes hydrophobic substances such as NPN but, once damaged, it can allow the entry of NPN to the phospholipid layer, resulting in prominent fluorescence. With Escherichia coli O157, Pseudomonas aeruginosa, and Salmonella typhimurium as test organisms and ethylenediaminetetraacetic acid and sodium hexametaphosphate as the model permeabilizers, quantitative and highly reproducible NPN uptake levels were obtained that differed characteristically between the test bacteria. Furthermore, citric acid was shown to be a potent permeabilizer at millimolar concentrations, its effect being partly (Ps. aeruginosa, Salm. typhimurium) or almost totally (E. coli O157) abolished by MgCl2, suggesting that part of the action occurs by chelation. Sodium citrate induced weak NPN uptake, which was totally abolished by MgCl2. In conclusion, the NPN uptake assay with the automated spectrofluorometer serves as a convenient method in analysing and quantifying the effects of external agents, including potential food preservatives, on Gram-negative bacteria.
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Cites
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Vaara, M. (1992) · Microbiological Reviews · doi:10.1128/mr.56.3.395-411.1992 · Cited by 1,879
Characterization of the action of selected essential oil components on Gram-negative bacteria
Helander, I. M., Alakomi, H.-L., Latva-Kala, K., Mattila-Sandholm, T., Pol, I., Smid, E. J., Gorris, L. G. M., & von Wright, A. (1998) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf980154m · Cited by 1,664
Self-citationFood preservation by hurdle technology
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Cellular and Molecular Biology
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Potential of lactic acid bacteria and novel antimicrobials against Gram-negative bacteria
Helander, I. M., von Wright, A., & Mattila-Sandholm, T.-M. (1997) · Trends in Food Science & Technology · doi:10.1016/s0924-2244(97)01030-3 · Cited by 306
Self-citationCompounds which increase the permeability of the Pseudomonas aeruginosa outer membrane
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Cited by
Chitosan disrupts the barrier properties of the outer membrane of Gram-negative bacteria
Helander, I. M., Nurmiaho-Lassila, E.-L., Ahvenainen, R., Rhoades, J., & Roller, S. (2001) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(01)00609-2 · Cited by 1,373
Self-citationLactic Acid Permeabilizes Gram-Negative Bacteria by Disrupting the Outer Membrane
Alakomi, H.-L., Skyttä, E., Saarela, M., Mattila-Sandholm, T., Latva-Kala, K., & Helander, I. M. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.5.2001-2005.2000 · Cited by 1,093
Self-citationOrganic acids as antimicrobials to control Salmonella in meat and poultry products
Mani-López, E., García, H. S., & López-Malo, A. (2012) · Food Research International · doi:10.1016/j.foodres.2011.04.043 · Cited by 615
Berry Phenolics: Antimicrobial Properties and Mechanisms of Action Against Severe Human Pathogens
Nohynek, L. J., Alakomi, H.-L., Kähkönen, M. P., Heinonen, M., Helander, I. M., Oksman-Caldentey, K.-M., & Puupponen-Pimiä, R. H. (2006) · Nutrition and Cancer · doi:10.1207/s15327914nc5401_4 · Cited by 537
Self-citationColistin kills bacteria by targeting lipopolysaccharide in the cytoplasmic membrane
Sabnis, A., Hagart, K. L., Klöckner, A., Becce, M., Evans, L. E., Furniss, R. C. D., Mavridou, D. A., Murphy, R., Stevens, M. M., Davies, J. C., et al. (2021) · eLife · doi:10.7554/elife.65836 · Cited by 389
Metadata
- Authors
- Helander, I. M., & Mattila-Sandholm, T.
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-02
- Volume
- 88
- Issue
- 2
- Pages
- 213-219
- ISSN
- 1364-5072 (ISSN-L)
- 1365-2672
- OpenAlex
- W1966478139
- Cited by
- 537
- References
- 24
- FWCI
- 2.78
- Type
- journal article
- Language
- en
- Open access
- closed
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