The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
How much effort is required to isolate nuclear microsatellites from plants?
Abstract
The attributes of codominance, reproducibility and high resolution have all contributed towards the current popularity of nuclear microsatellites as genetic markers in molecular ecological studies. One of their major drawbacks, however, is the development phase required to obtain working primers for a given study species. To facilitate project planning, we have reviewed the literature to quantify the workload involved in isolating nuclear microsatellites from plants. We highlight the attrition of loci at each stage in the process, and the average effort required to obtain 10 working microsatellite primer pairs.
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Cites
Polymorphism revealed by simple sequence repeats
Powell, W., Machray, G. C., & Provan, J. (1996) · Trends in Plant Science · doi:10.1016/1360-1385(96)86898-1 · Cited by 1,258
Self-citationSefc, K. M., Lopes, M. S., Mendonça, D., Santos, M. R. D., Machado, M. L. D. C., & Machado, A. D. C. (2000) · Molecular Ecology · doi:10.1046/j.1365-294x.2000.00954.x · Cited by 358
de la Rosa, R., James, C. M., & Tobutt, K. R. (2002) · Molecular Ecology Notes · doi:10.1046/j.1471-8286.2002.00217.x · Cited by 249
Dayanandan, S. D., Dole, J. A., Bawa, K. S., & Kesseli, R. (1999) · Molecular Ecology · doi:10.1046/j.1365-294x.1999.00735.x · Cited by 129
White, G. M., & Powell, W. (1997) · Molecular Ecology · doi:10.1046/j.1365-294x.1997.d01-216.x · Cited by 101
Self-citationMicrosatellite loci in eelgrassZostera marinareveal marked polymorphism within and among populations
Reusch, T. B. H., & Olsen, J. (1999) · Molecular Ecology · doi:10.1046/j.1365-294x.1999.00531.x · Cited by 92
Lefort, F., Brachet, S., Frascaria‐Lacoste, N., Edwards, K. J., & Douglas, G. C. (1999) · Molecular Ecology · doi:10.1046/j.1365-294x.1999.00655_8.x · Cited by 76
Polymorphic microsatellite loci in Mimulus guttatus and related species
Kelly, A. J., & Willis, J. H. (1998) · Molecular Ecology · doi:10.1046/j.1365-294x.1998.00328.x · Cited by 76
Microsatellite markers for heart of palm – Euterpe edulis and E. oleracea Mart. (Arecaceae)
Gaiotto, F. A., Brondani, R. P. V., & Grattapaglia, D. (2001) · Molecular Ecology Notes · doi:10.1046/j.1471-8278.2001.00036.x · Cited by 48
Isolation of microsatellite loci for paternity testing in Phillyrea angustifolia L. (Oleaceae)
Saumitou‐Laprade, P., Vassiliadis, C., Epplen, J. T., & Hardt, C. (2000) · Molecular Ecology · doi:10.1046/j.1365-294x.2000.00764-4.x · Cited by 18
Cited by
Somaclonal Variation in Plants: Causes and Detection Methods
Bairu, M. W., Aremu, A. O., & Van Staden, J. (2011) · Plant Growth Regulation · doi:10.1007/s10725-010-9554-x · Cited by 674
Tall fescue EST-SSR markers with transferability across several grass species
Saha, M. C., Mian, M. A. R., Eujayl, I., Zwonitzer, J. C., Wang, L., & May, G. D. (2004) · Theoretical and Applied Genetics · doi:10.1007/s00122-004-1681-1 · Cited by 319
Miah, G., Rafii, M., Ismail, M., Puteh, A., Rahim, H., Islam, K., & Latif, M. (2013) · International Journal of Molecular Sciences · doi:10.3390/ijms141122499 · Cited by 241
Metadata
- Authors
- Squirrell, J., Hollingsworth, P. M., Woodhead, M., Russell, J., Lowe, A. J., Gibby, M., & Powell, W.
- Venue
- Molecular Ecology
- Publisher
- Wiley
- Published
- 2003-04-25
- Volume
- 12
- Issue
- 6
- Pages
- 1339-1348
- ISSN
- 0962-1083 (ISSN-L)
- 1365-294X
- OpenAlex
- W2003235189
- Cited by
- 329
- References
- 10
- FWCI
- 6.70
- Type
- journal article
- Language
- en
- Open access
- closed
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- Squirrell, J., Hollingsworth, P. M., Woodhead, M., Russell, J., Lowe, A. J., Gibby, M., & Powell, W. (2003). How much effort is required to isolate nuclear microsatellites from plants? Molecular Ecology. https://doi.org/10.1046/j.1365-294x.2003.01825.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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