Cited by 78Bacterial Infections and VaccinesEscherichia coli research studiesMicrobial infections and disease research

Interactions of Neisseria meningitidis with cells of the human meninges

Hardy, Samantha J., Christodoulides, Myron, Weller, Roy O., Heckels, John E.·2000·Molecular Microbiology

Abstract

The interaction of Neisseria meningitidis with the meninges that surround and protect the brain is a pivotal event in the progression of bacterial meningitis. Two models of the human meninges were established in vitro, using (i) sections of fresh human brain and (ii) cultures of viable cells grown from human meningiomas. Neisseria meningitidis showed a specific predilection for binding to the leptomeninges and meningeal blood vessels in human brain and not to the cerebral cortex. There was a close correlation between the adherence of different Neisseria species to leptomeninges and cultured cells. The major ligand that mediated adherence was the pilus, and pilin variation modulated the interactions. The presence of Opa protein increased the association of Cap+ meningococci that expressed low-adhesive pili, but did not influence the association of high-adhesive pili. In contrast, Opc did not influence the adherence of Cap+ meningococci, whereas loss of capsule was associated with a more intimate interaction between the bacteria and the meningioma cell that was not apparent with Cap+ meningococci. There was no evidence of internalization of meningococci by meningioma cells in vitro, an observation that is consistent with the barrier properties of the leptomeninges to N. meningitidis observed in vivo.

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Crossref · 10.1046/j.1365-2958.2000.01923.x

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54 of 54 references verified0 citation errors · 0 not checkable

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  • All major meningitis causing bacteria can adhere strongly to meningeal fibroblasts and arachnoid barrier-like cells, and Group B Streptococcus bacteria cross the blood brain barrier by binding to vimentin on endothelial cells, which is also highly expressed by arachnoid barrier cells.

    “All major meningitis causing bacteria can adhere strongly to meningeal fibroblasts and arachnoid barrier-like cells”

    From Living on the Edge of the CNS: Meninges Cell Diversity in Health and Disease (2021) · cites this paper as Hardy et al., 2000

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Authors
Hardy, S. J., Christodoulides, M., Weller, R. O., & Heckels, J. E.
Affiliations
Southampton General Hospital (GB)
Corresponding author
Christodoulides, Myron
Venue
Molecular Microbiology
Publisher
Wiley
Published
2000-05
Volume
36
Issue
4
Pages
817-829
ISSN
  • 0950-382X (ISSN-L)
  • 1365-2958
OpenAlex
W2060390877
Cited by
78
References
54
Citations by year
2026: 5 · 2025: 2 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 4 · 2015: 6 · 2014: 3 · 2013: 3 · 2012: 5
FWCI
2.14
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • ePrints Soton (University of Southampton) (submittedVersion)
Funders
  • University of Liverpool
  • Medical Research Council
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
Neisseria meningitidis, bacterial meningitis, leptomeninges, Opa proteins, meningeal blood vessels, bacterial internalization, host-pathogen interaction
MeSH terms
Animals, Bacterial Adhesion, Brain, Humans, Meninges, Meningioma, Neisseria meningitidis, Species Specificity, Tumor Cells, Cultured, Microscopy, Confocal, Mice

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