Novel fungal transcriptional activators, Cmr1p of Colletotrichum lagenarium and Pig1p of Magnaporthe grisea , contain Cys2His2 zinc finger and Zn(II)2Cys6 binuclear cluster DNA‐binding motifs and regulate transcription of melanin biosynthesis genes in a developmentally specific manner
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Colletotrichum lagenarium and Magnaporthe grisea are plant pathogenic fungi that produce melanin during the appressorial differentiation stage of conidial germination and during the late stationary phase of mycelial growth. Here, we report the identification of genes for two unique transcription factors, CMR1 ( C olletotrichum m elanin r egulation) and PIG1 ( pig ment of Magnaporthe ), that are involved in melanin biosynthesis. Both Cmr1p and Pig1p contain two distinct DNA‐binding motifs, a Cys2His2 zinc finger motif and a Zn(II)2Cys6 binuclear cluster motif. The presence of both these motifs in a single transcriptional regulatory protein is unique among known eukaryotic transcription factors. Deletion of CMR1 in C. lagenarium caused a defect in mycelial melanization, but not in appressorial melanization. Also, cmr1 Δ mutants do not express the melanin biosynthetic structural genes SCD1 and THR1 during mycelial melanization, although the expression of these two genes was not affected during appressorial melanization.
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Δcmr1 mutants show impaired expression of the melanin biosynthetic structural genes SCD1 and THR1 during melanization, thereby affecting melanin biosynthesis.
“Additionally, Δcmr1 mutants show impaired expression of the melanin biosynthetic structural genes SCD1 and THR1 during melanization, thereby affecting melanin biosynthesis [53].”
From Fungal Melanin in Plant Pathogens: Complex Biosynthesis Pathways and Diverse Biological Functions (2025) · cites this paper as [53]
The Discussion (p. 949) says Cmr1p is essential for SCD1 and THR1 transcription during mycelial melanization, supporting the reported impairment and its effect on melanin biosynthesis. The claim omits that this is developmentally specific: the same passage says those transcripts accumulate and melanized appressoria form during appressorium differentiation in Δcmr1 mutants. (LLM, conf=0.86)
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A Fungal Family of Transcriptional Regulators: the Zinc Cluster Proteins
MacPherson, S., Larochelle, M., & Turcotte, B. (2006) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00015-06 · Cited by 612 · see citing works
Fungal Melanin in Plant Pathogens: Complex Biosynthesis Pathways and Diverse Biological Functions
Jia, H., Liu, N., Zhang, L., Li, P., Meng, Y., Yuan, W., Li, H., Tantai, D., Qu, Q., Cao, Z., et al. (2025) · Plants · doi:10.3390/plants14142121 · Cited by 18 · see citing works
- Authors
- Tsuji, G., Kenmochi, Y., Takano, Y., Sweigard, J., Farrall, L., Furusawa, I., Horino, O., & Kubo, Y.
- Affiliations
- Kyoto Prefectural University (JP)
- Kyoto University (JP)
- DuPont (United States) (US)
- Corresponding author
- Kubo, Yasuyuki
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2000-12
- Volume
- 38
- Issue
- 5
- Pages
- 940-954
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2135968016
- Cited by
- 172
- References
- 0
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 13 · 2023: 11 · 2022: 16 · 2021: 12 · 2020: 7 · 2019: 7 · 2018: 7 · 2017: 8 · 2016: 3 · 2015: 6 · 2014: 10 · 2013: 8 · 2012: 4
- FWCI
- 1.36
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (09460031, 12052219)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Plant Pathogens and Fungal Diseases
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- Colletotrichum lagenarium, Magnaporthe grisea, melanogenesis, transcription factors, C2H2 zinc finger, DNA-binding motifs, stearoyl-CoA desaturase 1, fungal development, plant pathogenic fungi
- MeSH terms
- Amino Acid Sequence, Base Sequence, Binding Sites, DNA, DNA-Binding Proteins, Fungal Proteins, Melanins, Molecular Sequence Data, Phylogeny, Transcription Factors, Transcription, Genetic, Trans-Activators, Gene Expression Regulation, Fungal, Zinc Fingers, Sequence Homology, Amino Acid, DNA, Complementary, Gene Expression Regulation, Developmental, Magnaporthe, Colletotrichum, Amino Acid Motifs
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