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Cited by 72Bacterial Genetics and BiotechnologyBiochemical and Molecular ResearchLegume Nitrogen Fixing Symbiosis

The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.

Physiological role of the GlnK signal transduction protein of Escherichia coli: survival of nitrogen starvation

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Blauwkamp, Timothy A., Ninfa, Alexander J.·2002·Molecular Microbiology
DOI 10.1046/j.1365-2958.2002.03153.x
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Abstract

Summary: Escherichia coli contains two PII‐like signal trans‐duction proteins, PII and GlnK, involved in nitrogen assimilation. We examined the roles of PII and GlnK in controlling expression of glnALG , glnK and nac during the transition from growth on ammonia to nitrogen starvation and vice versa. The PII protein exclusively controlled glnALG expression in cells adapted to growth on ammonia, but was unable to limit nac and glnK expression under conditions of nitrogen starvation. Conversely, GlnK was unable to limit glnALG expression in cells adapted to growth on ammonia, but was required to limit expression of the glnK and nac promoters during nitrogen starvation. In the absence of GlnK, very high expression of the glnK and nac promoters occurred in nitrogen‐starved cells, and the cells did not reduce glnK and nac expression when given ammonia. Thus, one specific role of GlnK is to regulate the expression of Ntr genes during nitrogen starvation. GlnK also had a dramatic effect on the ability of cells to survive nitrogen starvation and resume rapid growth when fed ammonia. After being nitrogen starved for as little as 10 h, cells lacking GlnK were unable to resume rapid growth when given ammonia. In contrast, wild‐type cells that were starved immediately resumed rapid growth when fed ammonia. Cells lacking GlnK also showed faster loss of viability during extended nitrogen starvation relative to wild‐type cells. This complex phenotype resulted partly from the requirement for GlnK to regulate nac expression; deletion of nac restored wild‐type growth rates after ammonia starvation and refeeding to cells lacking GlnK, but did not improve viability during nitrogen starvation. The specific roles of GlnK during nitrogen starvation were not the result of a distinct function of the protein, as expression of PII from the glnK promoter in cells lacking GlnK restored the wild‐type phenotypes.

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  1. 10.1128/MMBR.59.4.604-622.1995 · DOI
  2. Silhavy T.J., 1984, Experi‐ments with Gene Fusions., 107

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Metadata

Authors
Blauwkamp, T. A., & Ninfa, A. J.
Affiliations
University of Michigan (US)
Corresponding author
Ninfa, Alexander J.
ORCID
Blauwkamp, Timothy A.
Venue
Molecular Microbiology
Publisher
Wiley
Published
2002-10
Volume
46
Issue
1
Pages
203-214
ISSN
  • 0950-382X (ISSN-L)
  • 1365-2958
DOI
10.1046/j.1365-2958.2002.03153.x
OpenAlex
W2170000137
Cited by
72
References
28
Citations by year
2026: 1 · 2025: 4 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 4 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 1 · 2016: 1 · 2013: 5 · 2012: 2
FWCI
2.47
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 2 d)
Locations
  • Molecular Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • Deep Blue (University of Michigan) (submittedVersion)
Funders
  • National Institutes of Health (5r01gm057393-04)
  • National Institute of General Medical Sciences (GM57393, T32GM145304)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Legume Nitrogen Fixing Symbiosis
Keywords
GlnK, PII protein, signal transduction, nitrogen starvation, nitrogen assimilation, gene expression regulation, Escherichia coli, cell viability
MeSH terms
Ammonia, Bacterial Proteins, Carrier Proteins, Culture Media, Escherichia coli, Mutation, Nitrogen, Phenotype, Promoter Regions, Genetic, Transcription, Genetic, Signal Transduction, Gene Expression Regulation, Bacterial, PII Nitrogen Regulatory Proteins

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