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PCR‐based detection of Colletotrichum acutatum on strawberry
Abstract
An oligonucleotide primer ( Ca Int 2) was synthesized from the variable internal transcribed spacer (ITS) 1 region of ribosomal DNA (rDNA) from Colletotrichum acutatum . PCR with primers Ca Int2 and ITS4 (from a conserved sequence of the rDNA) amplified a 490 bp fragment from several isolates of C. acutatum but not from other members of the genus Colletotrichum . Amplification of this fragment was achieved from 100 fg of fungal DNA. These primers amplified a fragment of the same size from DNA extracted from strawberry tissues infected by C. acutatum . Southern hybridization analysis confirmed the 490 bp fragment from C. acutatum DNA and infected strawberry to be identical. The species‐specific primer ( Ca Int2) developed in this work could be used for the accurate identification of C. acutatum and its detection on other host plants.
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Citations
Cited by
Molecular diagnostics for fungal plant pathogens
McCartney, H. A., Foster, S. J., Fraaije, B. A., & Ward, E. (2003) · Pest Management Science · doi:10.1002/ps.575 · Cited by 401
SREENIVASAPRASAD, S., & TALHINHAS, P. (2005) · Molecular Plant Pathology · doi:10.1111/j.1364-3703.2005.00291.x · Cited by 176
Self-citationAdaskaveg, J. E., & Hartin, R. J. (1997) · Phytopathology® · doi:10.1094/phyto.1997.87.9.979 · Cited by 165
Characterization of Colletotrichum Species Causing Bitter Rot of Apple in Kentucky Orchards
Munir, M., Amsden, B., Dixon, E., Vaillancourt, L., & Gauthier, N. A. W. (2016) · Plant Disease · doi:10.1094/pdis-10-15-1144-re · Cited by 126
Whitelaw‐Weckert, M. A., Curtin, S. J., Huang, R., Steel, C. C., Blanchard, C. L., & Roffey, P. E. (2007) · Plant Pathology · doi:10.1111/j.1365-3059.2007.01569.x · Cited by 110
Metadata
- Authors
- SREENIVASAPRASAD, S., SHARADA, K., BROWN, A. E., & MILLS, P. R.
- Venue
- Plant Pathology
- Publisher
- Wiley
- Published
- 1996-08
- Volume
- 45
- Issue
- 4
- Pages
- 650-655
- ISSN
- 0032-0862 (ISSN-L)
- 1365-3059
- OpenAlex
- W2135437376
- Cited by
- 161
- References
- 0
- FWCI
- 1.39
- Type
- journal article
- Language
- en
- Open access
- closed
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- SREENIVASAPRASAD, S., SHARADA, K., BROWN, A. E., & MILLS, P. R. (1996). PCR‐based detection of Colletotrichum acutatum on strawberry. Plant Pathology. https://doi.org/10.1046/j.1365-3059.1996.d01-3.x
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