The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis
Open accessAbstract
Rice (Oryza sativa L.), the major food staple for more than two billion people, contains neither β‐carotene (provitamin A) nor C 40 carotenoid precursors thereof in its endosperm. To improve the nutritional value of rice, genetic engineering was chosen as a means to introduce the ability to make β‐carotene into rice endosperm tissue. Investigation of the biochemical properties of immature rice endosperm using [14 C]‐labelled substrates revealed the presence of geranyl geranyl diphosphate, the C 20 general isoprenoid precursor necessary for C 40 carotenoid biosynthesis. Phytoene synthase, which condenses two molecules of geranyl geranyl diphosphate, is the first of four specific enzymes necessary for β‐carotene biosynthesis in plants. Therefore, the Japonica rice model variety Taipei 309 was transformed by microprojectile bombardment with a cDNA coding for phytoene synthase from daffodil (Narcissus pseudonarcissus) under the control of either a constitutive or an endosperm‐specific promoter. In transgenic rice plants, the daffodil enzyme is active, as measured by the in vivo accumulation of phytoene in rice endosperm. Thus, it is demonstrated for the first time that it is in principle possible to engineer a critical step in provitamin A biosynthesis in a non‐photosynthetic, carotenoid‐lacking plant tissue. These results have important implications for longterm prospects of overcoming worldwide vitamin A deficiency.
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Cites
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Improving the nutritional value of Golden Rice through increased pro-vitamin A content
Paine, J. A., Shipton, C. A., Chaggar, S., Howells, R. M., Kennedy, M. J., Vernon, G., Wright, S. Y., Hinchliffe, E., Adams, J. L., Silverstone, A. L., et al. (2005) · Nature Biotechnology · doi:10.1038/nbt1082 · Cited by 1,457
The biosynthesis and nutritional uses of carotenoids
Fraser, P. (2004) · Progress in Lipid Research · doi:10.1016/j.plipres.2003.10.002 · Cited by 1,375
Carotenoid Metabolism in Plants
Nisar, N., Li, L., Lu, S., Khin, N. C., & Pogson, B. J. (2015) · Molecular Plant · doi:10.1016/j.molp.2014.12.007 · Cited by 1,255
Biogenesis, molecular regulation and function of plant isoprenoids.
Bouvier, F., Rahier, A., & Camara, B. (2005) · Progress in Lipid Research · doi:10.1016/j.plipres.2005.09.003 · Cited by 498
The Epigenome and Transcriptional Dynamics of Fruit Ripening
Giovannoni, J., Nguyen, C., Ampofo, B., Zhong, S., & Fei, Z. (2017) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-042916-040906 · Cited by 474
Metadata
- Authors
- Burkhardt, P. K., Beyer, P., Wünn, J., Klöti, A., Armstrong, G. A., Schledz, M., von Lintig, J., & Potrykus, I.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 1997-05
- Volume
- 11
- Issue
- 5
- Pages
- 1071-1078
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2103966436
- Cited by
- 375
- References
- 22
- FWCI
- 5.29
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis. https://app.cochat.ai/papers/10.1046/j.1365-313x.1997.11051071.x
- The paper
- Burkhardt, P. K., Beyer, P., Wünn, J., Klöti, A., Armstrong, G. A., Schledz, M., von Lintig, J., & Potrykus, I. (1997). Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis. The Plant Journal. https://doi.org/10.1046/j.1365-313x.1997.11051071.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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