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Rapid isolation of high molecular weight plant DNA
Open accessAbstract
A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA (i.e. nuclear, chloroplast, and mitochondrial DNA). The technique is ideal for the rapid isolation of small amounts of DNA from many different species and is also useful for large scale isolations.
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Citations
Cited by
Sheehan, J., Dunahay, T., Benemann, J., & Roessler, P. (1998) · doi:10.2172/15003040 · Cited by 1,753
Caccavo, F., Lonergan, D. J., Lovley, D. R., Davis, M., Stolz, J. F., & McInerney, M. J. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.10.3752-3759.1994 · Cited by 896
Li, W., Hartung, J. S., & Levy, L. (2006) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2005.10.018 · Cited by 873
A CRISPR–Cpf1 system for efficient genome editing and transcriptional repression in plants
Tang, X., Lowder, L. G., Zhang, T., Malzahn, A. A., Zheng, X., Voytas, D. F., Zhong, Z., Chen, Y., Ren, Q., Li, Q., et al. (2017) · Nature Plants · doi:10.1038/nplants.2017.18 · Cited by 616
Massive haplotypes underlie ecotypic differentiation in sunflowers
Todesco, M., Owens, G. L., Bercovich, N., Légaré, J.-S., Soudi, S., Burge, D. O., Huang, K., Ostevik, K. L., Drummond, E. B. M., Imerovski, I., et al. (2020) · Nature · doi:10.1038/s41586-020-2467-6 · Cited by 513
Metadata
- Authors
- Murray, M. G., & Thompson, W. F.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1980
- Volume
- 8
- Issue
- 19
- Pages
- 4321-4326
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2082091267
- Cited by
- 12,098
- References
- 0
- FWCI
- 2.09
- Type
- journal article
- Language
- en
- Open access
- green
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- Murray, M. G., & Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research. https://doi.org/10.1093/nar/8.19.4321
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
