Fluconazole toxicity is independent of oxidative stress and apoptotic effector mechanisms in Saccharomyces cerevisiae
Abstract
Summary Fluconazole toxicity was previously shown to require intact mitochondria in Saccharomyces cerevisiae , however, the mechanism of mitochondrial‐dependent azole toxicity is unclear. Here we show that fluconazole toxicity is not attenuated by the overexpression of the mitochondrially‐acting human antiapoptotic protein Bcl‐2 nor is it increased by the presence of the oxidating agents in S. cerevisiae . Our data further support the notion that mitochondrial toxicity in the presence of azoles is modulated by the conversion of ergosterol precursors to toxic sterols.
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Azoles exhibit a certain degree of toxicity that has already been demonstrated.
“Nevertheless, in comparison, azoles exhibit a certain degree of toxicity that has been already demonstrated.5,19”
S. cerevisiae has been described as an attractive experimental system for studying azole toxicity mechanisms.
“With respect to S. cerevisiae, it has been also described as an attractive experimental system for studying azole toxicity mechanisms.19”
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- Authors
- Kontoyiannis, D. P., & Murray, P. J.
- Affiliations
- The University of Texas MD Anderson Cancer Center (US)
- St. Jude Children's Research Hospital (US)
- Corresponding author
- Kontoyiannis, D. P.
- Venue
- Mycoses
- Publisher
- Wiley
- Published
- 2003-06
- Volume
- 46
- Issue
- 5-6
- Pages
- 183-186
- ISSN
- 0027-5557 (ISSN-L)
- 0933-7407
- 1439-0507
- OpenAlex
- W2135828518
- Cited by
- 8
- References
- 12
- Citations by year
- 2026: 1 · 2012: 1
- FWCI
- 0.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Mycoses (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- azole antifungals, mitochondrial toxicity, oxidative stress, apoptosis, Bcl-2, Saccharomyces cerevisiae
- MeSH terms
- Mitochondria, Saccharomyces cerevisiae, Transfection, Fluconazole, Apoptosis, Oxidative Stress, Proto-Oncogene Proteins c-bcl-2
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