CoChat
Sign Up
Cited by 198Legionella and Acanthamoeba researchSlime Mold and Myxomycetes ResearchAmoebic Infections and Treatments

The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.

Dictyostelium discoideum: a new host model system for intracellular pathogens of the genus Legionella

Hagele, Sonja, Kohler, Rolf, Merkert, Hilde, Schleicher, Michael, Hacker, Jorg, Steinert, Michael·2000·Cellular Microbiology
DOI 10.1046/j.1462-5822.2000.00044.x
Open this paper in CoChat — free account

Abstract

The soil amoeba Dictyostelium discoideum is a haploid eukaryote that, upon starvation, aggregates and enters a developmental cycle to produce fruiting bodies. In this study, we infected single-cell stages of D. discoideum with different Legionella species. Intracellular growth of Legionella in this new host system was compared with their growth in the natural host Acanthamoeba castellanii. Transmission electron microscopy of infected D. discoideum cells revealed that legionellae reside within the phagosome. Using confocal microscopy, it was observed that replicating, intracellular, green fluorescent protein (GFP)-tagged legionellae rarely co-localized with fluorescent antibodies directed against the lysosomal protein DdLIMP of D. discoideum. This indicates that the bacteria inhibit the fusion of phagosomes and lysosomes in this particular host system. In addition, Legionella infection of D. discoideum inhibited the differentiation of the host into the multicellular fruiting stage. Co-culture studies with profilin-minus D. discoideum mutants and Legionella resulted in higher rates of infection when compared with infections of wild-type amoebae. Because the amoebae are amenable to genetic manipulation as a result of their haploid genome and because a number of cellular markers are available, we show for the first time that D. discoideum is a valuable model system for studying intracellular pathogenesis of microbial pathogens.

Overview

Integrity signals

Registry

Crossref · 10.1046/j.1462-5822.2000.00044.x

Cites

35 of 35 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 1

  1. 10.1016/S0378-1097(98)00463-7 · DOI

Cited by

Sorted by citations

See all 198

Related

See all

Metadata

Authors
Hagele, S., Kohler, R., Merkert, H., Schleicher, M., Hacker, J., & Steinert, M.
Affiliations
  • University of Würzburg (DE)
  • Ludwig-Maximilians-Universität München (DE)
Corresponding author
Steinert, Michael
ORCID
Steinert, Michael
Venue
Cellular Microbiology
Publisher
Hindawi Limited
Published
2000-04
Volume
2
Issue
2
Pages
165-171
ISSN
  • 1462-5814 (ISSN-L)
  • 1462-5822
DOI
10.1046/j.1462-5822.2000.00044.x
OpenAlex
W2035842134
Cited by
198
References
35
Citations by year
2026: 2 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 5 · 2020: 5 · 2019: 7 · 2018: 13 · 2017: 11 · 2016: 1 · 2015: 5 · 2014: 16 · 2013: 15 · 2012: 6
FWCI
5.25
Type
journal article
Language
en
Open access
closed
Licence
doi.wiley.com/10.1002/tdm_license_1.1 (tdm, 5631 d)
Locations
  • Cellular Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Legionella and Acanthamoeba research
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Slime Mold and Myxomycetes Research
  • Health Sciences › Medicine › Infectious Diseases › Amoebic Infections and Treatments
Keywords
Dictyostelium discoideum, Legionella, intracellular pathogens, host-pathogen interaction, profilin, Acanthamoeba castellanii, intracellular bacterial growth
MeSH terms
Acanthamoeba, Animals, Dictyostelium, Disease Models, Animal, Flow Cytometry, Humans, Legionella, Legionellosis, In Situ Hybridization, Microscopy, Confocal

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review