The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Ascorbic acid increases the yield of dopaminergic neurons derived from basic fibroblast growth factor expanded mesencephalic precursors
Abstract
CNS precursors derived from E12 rat mesencephalon proliferate in the presence of basic fibroblast growth factor and differentiate in vitro into functional dopaminergic neurons, which upon transplantation alleviate behavioral symptoms in a rat model of Parkinson's disease. Here we show that the efficiency of dopaminergic differentiation decreases in the mesencephalic precursors that were proliferated or passaged for extended periods in vitro. Ascorbic acid treatment restored dopaminergic differentiation in these precursors and led to a greater than 10‐fold increase in dopamine neuron yield compared with untreated cultures. The effect of ascorbic acid was stereospecific and could not be mimicked by any other antioxidants. The expression of sodium‐dependent vitamin C transporter, a recently identified stereospecific ascorbic acid transporter, was maintained in mesencephalic precursors for extended in vitro periods. Pre‐treatment of in vitro expanded mesencephalic precursors with ascorbic acid might facilitate the large‐scale generation of dopaminergic neurons for clinical transplantation.
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A family of mammalian Na+-dependent L-ascorbic acid transporters
Tsukaguchi, H., Tokui, T., Mackenzie, B., Berger, U. V., Chen, X.-Z., Wang, Y., Brubaker, R. F., & Hediger, M. A. (1999) · Nature · doi:10.1038/19986 · Cited by 882
8-Hydroxydeoxyguanosine and 8-hydroxyguanine as biomarkers of oxidative DNA damage
Helbock, H. J., Beckman, K. B., & Ames, B. N. (1999) · Methods in Enzymology · doi:10.1016/s0076-6879(99)00123-8 · Cited by 215
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Lindvall, O., Kokaia, Z., & Martinez-Serrano, A. (2004) · Nature Medicine · doi:10.1038/nm1064 · Cited by 915
Barberi, T., Klivenyi, P., Calingasan, N. Y., Lee, H., Kawamata, H., Loonam, K., Perrier, A. L., Bruses, J., Rubio, M. E., Topf, N., et al. (2003) · Nature Biotechnology · doi:10.1038/nbt870 · Cited by 623
Self-citationDuarte, T. L., & Lunec, J. (2005) · Free Radical Research · doi:10.1080/10715760500104025 · Cited by 374
Regionalization and fate specification in neurospheres: the role of Olig2 and Pax6
Hack, M. A., Sugimori, M., Lundberg, C., Nakafuku, M., & Götz, M. (2004) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2003.12.012 · Cited by 269
Vitamin C in Health and Disease: Its Role in the Metabolism of Cells and Redox State in the Brain
Figueroa-Méndez, R., & Rivas-Arancibia, S. (2015) · Frontiers in Physiology · doi:10.3389/fphys.2015.00397 · Cited by 257
Metadata
- Authors
- Yan, J., Studer, L., & McKay, R. D. G.
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 2001-01
- Volume
- 76
- Issue
- 1
- Pages
- 307-311
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W1849161055
- Cited by
- 172
- References
- 2
- FWCI
- 5.21
- Type
- journal article
- Language
- en
- Open access
- closed
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- Yan, J., Studer, L., & McKay, R. D. G. (2001). Ascorbic acid increases the yield of dopaminergic neurons derived from basic fibroblast growth factor expanded mesencephalic precursors. Journal of Neurochemistry. https://doi.org/10.1046/j.1471-4159.2001.00073.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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