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Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity
Abstract
A comparative study on the antimicrobial properties of extracts from medicinal plants obtained by two different methods was carried out. The screening of the antimicrobial activity of extracts from six plants was conducted by a disc diffusion test against Gram-positive, -negative and fungal organisms. The most active extracts (inhibition diameter >/=12 mm) were assayed for the minimum inhibitory concentration and submitted to phytochemical screening by thin-layer chromatography and bioautography. The results obtained indicate that the diethyl ether extracts were the most efficient antimicrobial compounds. The activity was more pronounced against Gram-positive and fungal organisms than against Gram-negative bacteria. Bioautography showed that the antimicrobial activity was probably due to flavonoids and terpenes.
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Citations
Cites
Antimicrobial activity of essential oils and other plant extracts
Hammer, K. A., Carson, C. F., & Riley, T. V. (1999) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.1999.00780.x · Cited by 2,560
Cited by
Screening methods to determine antibacterial activity of natural products
Valgas, C., Souza, S. M. D., Smânia, E. F. A., & Smânia Jr., A. (2007) · Brazilian Journal of Microbiology · doi:10.1590/s1517-83822007000200034 · Cited by 1,403
Ncube, N. S., Afolayan, A. J., & Okoh, A. I. (2008) · African Journal of Biotechnology · doi:10.5897/ajb07.613 · Cited by 821
Antibacterial activity of extracts from some edible plants commonly consumed in Asia
Alzoreky, N. S., & Nakahara, K. (2003) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(02)00169-1 · Cited by 615
Medicinal plants of the Meinit ethnic group of Ethiopia: An ethnobotanical study
Giday, M., Asfaw, Z., & Woldu, Z. (2009) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2009.05.009 · Cited by 579
Rehana, D., Mahendiran, D., Kumar, R. S., & Rahiman, A. K. (2017) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2017.02.101 · Cited by 494
Metadata
- Authors
- Nostro, A., Germano, M. P., D'Angelo, V., Marino, A., & Cannatelli, M. A.
- Venue
- Letters in Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-05
- Volume
- 30
- Issue
- 5
- Pages
- 379-384
- ISSN
- 0266-8254 (ISSN-L)
- 1365-2673
- OpenAlex
- W2000362546
- Cited by
- 858
- References
- 1
- FWCI
- 4.77
- Type
- journal article
- Language
- en
- Open access
- closed
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- Nostro, A., Germano, M. P., D'Angelo, V., Marino, A., & Cannatelli, M. A. (2000). Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in Applied Microbiology. https://doi.org/10.1046/j.1472-765x.2000.00731.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
