Promoter/Enhancer Cassettes for Keratinocyte Gene Therapy
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The CMV enhancer/β-actin promoter element is active in stem cells and in many epithelial tissues, including skin.
“To drive expression of TERT, we chose a CMV enhancer/β-actin promoter (actin-rtTA+) because this element was previously shown to be active in stem cells23 and in many epithelial tissues, including skin24,25.”
From Conditional telomerase induction causes proliferation of hair follicle stem cells (2005) · cites this paper as [25]
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- Authors
- Sawamura, D., Meng, X., Ina, S., Nakano, H., Tamai, K., Nomura, K., Hanada, K., Hashimoto, I., & Miyazaki, J.-I.
- Affiliations
- Hirosaki University (JP)
- The University of Osaka (JP)
- Corresponding author
- Sawamura, Daisuke
- Venue
- Journal of Investigative Dermatology
- Publisher
- Elsevier BV
- Published
- 1999-05
- Volume
- 112
- Issue
- 5
- Pages
- 828-830
- ISSN
- 0022-202X (ISSN-L)
- OpenAlex
- W2039800359
- Cited by
- 18
- References
- 13
- FWCI
- 1.92
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Investigative Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Keywords
- transcriptional regulation, lacZ reporter, beta-galactosidase expression, SV40 polyadenylation signal, cytomegalovirus immediate early promoter, Rous sarcoma virus promoter, transgene expression
- MeSH terms
- Animals, beta-Galactosidase, Cells, Cultured, Enhancer Elements, Genetic, Humans, Plasmids, Promoter Regions, Genetic, Genetic Therapy, Keratinocytes, Polymerase Chain Reaction, Genes, Reporter, Mice, Rats
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