Cited by 101T-cell and B-cell ImmunologyPsoriasis: Treatment and PathogenesisDermatology and Skin Diseases

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The TCR δ Repertoire in Normal Human Skin is Restricted and Distinct from the TCR δ Repertoire in the Peripheral Blood

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Holtmeier, Wolfgang, Pfänder, Michael, Hennemann, Andreas, Caspary, Wolfgang F., Zollner, Thomas M., Kaufmann, Roland·2001·Journal of Investigative Dermatology

DOI 10.1046/j.1523-1747.2001.01250.x

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Abstract

The skin and the intestinal mucosa form surfaces to external environments and share similarities in anatomic structure and immunologic defense. In healthy humans, intestinal gamma/delta T cells express a highly restricted gamma/delta T cell receptor repertoire whereas gamma/delta T cells of the skin were thought to express a polyclonal repertoire. Herein we report, using complementarity-determining region 3 size spectratyping and nucleotide sequencing of T cell receptor DV1 and DV2 rearrangements, that the human skin is also composed of clonally expanded gamma/delta T cells that are widely distributed. Identical complementarity-determining region 3 profiles and T cell receptor delta rearrangements were found in two separate skin samples that were obtained as far as 2-10 cm apart. Furthermore, analysis of peripheral blood mononuclear cells of these subjects clearly demonstrated that the skin harbors a unique population of gamma/delta T cells that is distinct from that in the peripheral blood. In addition comparable data were obtained irrespective of whether DNA or RNA was analyzed, indicating that the observed oligoclonality is not secondary to the expression of large amounts of mRNA from a few activated cells. Thus, gamma/delta T cells of the skin and the intestine both express an oligoclonal repertoire that enables them to respond to a variety of deleterious antigens without the need for diverse T cell receptors, possibly by recognition of stress-induced self-antigens or of conserved foreign antigens.

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Sequences of proteins of immunological interest
Kabat, B. E. A. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90805-4 · Cited by 3,363
Simplified mammalian DNA isolation procedure
Laird, P. W., Zijderveld, A., Linders, K., Rudnicki, M. A., Jaenisch, R., & Berns, A. J. M. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.15.4293 · Cited by 1,497
Recognition of Stress-Induced MHC Molecules by Intestinal Epithelial γδ T Cells
Groh, V., Steinle, A., Bauer, S., & Spies, T. A. (1998) · Science · doi:10.1126/science.279.5357.1737 · Cited by 1,142
γδ Cells: A Right Time and a Right Place for a Conserved Third Way of Protection
Hayday, A. (2000) · Annual Review of Immunology · doi:10.1146/annurev.immunol.18.1.975 · Cited by 1,134
Broad tumor-associated expression and recognition by tumor-derived γδ T cells of MICA and MICB
Groh, V., Rhinehart, R. L., Secrist, H., Bauer, S., Grabstein, K. H., & Spies, T. A. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.12.6879 · Cited by 1,026
Lymphocytes bearing antigen-specific γδ T-cell receptors accumulate in human infectious disease lesions
Modlin, R. L., Pirmez, C., Hofman, F. M., Torigian, V. K., Uyemura, K., Rea, T. H., Bloom, B. R., & Brenner, M. B. (1989) · Nature · doi:10.1038/339544a0 · Cited by 573
Limited diversity of γδ antigen receptor genes of thy-1+ dendritic epidermal cells
Asarnow, D. M., Kuziel, W. A., Bonyhad, M., Tigelaar, R. E., Tucker, P. W., & Allison, J. P. (1988) · Cell · doi:10.1016/0092-8674(88)90139-0 · Cited by 517
CDR3 length in antigen-specific immune receptors.
Rock, E. P., Sibbald, P. R., Davis, M. M., & Chien, Y. (1994) · The Journal of Experimental Medicine · doi:10.1084/jem.179.1.323 · Cited by 515
The anatomical basis of intestinal immunity
Mowat, A. M., & Viney, J. L. (1997) · Immunological Reviews · doi:10.1111/j.1600-065x.1997.tb00966.x · Cited by 493
Inflammatory Skin Diseases, T Cells, and Immune Surveillance
Robert, C., & Kupper, T. S. (1999) · New England Journal of Medicine · doi:10.1056/nejm199912093412407 · Cited by 474
Recognition of Self Antigens by Skin-Derived T Cells with Invariant γδ Antigen Receptors
Havran, W. L., Chien, Y., & Allison, J. P. (1991) · Science · doi:10.1126/science.1828619 · Cited by 359
Distinct antigen receptor repertoires of two classes of murine epithelium-associated T cells
Asarnow, D. M., Goodman, T. G., Lefrançois, L., & Allison, J. P. (1989) · Nature · doi:10.1038/341060a0 · Cited by 212
Human epidermal T cells predominantly belong to the lineage expressing alpha/beta T cell receptor.
Foster, C. A., Yokozeki, H., Rappersberger, K., Koning, F., Volc-Platzer, B., Rieger, A., Coligan, J. E., Wolff, K., & Stingl, G. (1990) · The Journal of experimental medicine · doi:10.1084/jem.171.4.997 · Cited by 182
Human intestinal intraepithelial lymphocytes are derived from a limited number of T cell clones that utilize multiple V beta T cell receptor genes
Blumberg, R. S., Yockey, C. E., Gross, G. G., Ebert, E. C., & Balk, S. P. (1993) · The Journal of Immunology · doi:10.4049/jimmunol.150.11.5144 · Cited by 172
Thy-1+ dendritic epidermal cells express T3 antigen and the T-cell receptor gamma chain.
Stingl, G., Koning, F., Yamada, H., Yokoyama, W. M., Tschachler, E., Bluestone, J. A., Steiner, G. E., Samelson, L. E., Lew, A. M., & Coligan, J. E. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.13.4586 · Cited by 157
T-Cell Receptor γδ Bearing Cells in Normal Human Skin
Bos, J. D., Teunissen, M. B. M., Cairo, I., Krieg, S. R., Kapsenberg, M. L., Das, P. K., & Borst, J. G. (1990) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12873333 · Cited by 132
The γδ T-cell bridge: Linking innate and acquired immunity
Mak, T. W., & Ferrick, D. A. (1998) · Nature Medicine · doi:10.1038/nm0798-764 · Cited by 117
The V delta 1 T cell receptor repertoire in human small intestine and colon.
Chowers, Y., Holtmeier, W., Harwood, J. I., Morzycka-Wroblewska, E., & Kagnoff, M. F. (1994) · The Journal of Experimental Medicine · doi:10.1084/jem.180.1.183 · Cited by 97
Self-citation
Evidence for clonal selection of gamma/delta T cells in response to a human pathogen.
Uyemura, K., Deans, R. J., Band, H., Ohmen, J. D., Panchamoorthy, G., Morita, C. T., Rea, T. H., & Modlin, R. L. (1991) · The Journal of Experimental Medicine · doi:10.1084/jem.174.3.683 · Cited by 89
Non‐random expression of T cell receptor γ and δ variable gene segments in functional T lymphocyte clones from human peripheral blood
Borst, J. G., Wicherink, A., van Dongen, J. J. M., de Vries, E., Comans-Bitter, W. M., Wassenaar, F., & van den Elsen, P. J. (1989) · European Journal of Immunology · doi:10.1002/eji.1830190907 · Cited by 88
Distribution of dominant T cell receptor beta chains in human intestinal mucosa.
Gross, G. G., Schwartz, V., Stevens, C., Ebert, E. C., Blumberg, R. S., & Balk, S. P. (1994) · The Journal of Experimental Medicine · doi:10.1084/jem.180.4.1337 · Cited by 87
T-Cell Receptor-γ/δ Bearing Lymphocytes in Normal and Inflammatory Human Skin
Dupuy, P., Heslan, M., Fraitag, S., Hercend, T., Dubertret, L., & Bagot, M. (1990) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12874626 · Cited by 76
T-cell receptor V beta expression in normal human skin.
Dunn, D. A., Gadenne, A. S., Simha, S., Lerner, E. A., Bigby, M. E., & Bleicher, P. A. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.4.1267 · Cited by 72
Identification of MART-1-specific T-cell receptors: T cells utilizing distinct T-cell receptor variable and joining regions recognize the same tumor epitope.
Cole, D. J., Weil, D. P., Shamamian, P. E., Rivoltini, L., Kawakami, Y., Topalian, S. L., Jennings, C., Eliyahu, S., Rosenberg, S. A., & Nishimura, M. I. (1994) · PubMed · Cited by 63
TCR γ/δ + Dendritic Epidermal T Cells as Constituents of Skin-Associated Lymphoid Tissue
Tigelaar, R. E., Lewis, J. M., & Bergstresser, P. R. (1990) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12875138 · Cited by 62
The TCR-δ repertoire in human intestine undergoes characteristic changes during fetal to adult development
Holtmeier, W., Witthöft, T., Hennemann, A., Winter, H. S., & Kagnoff, M. F. (1997) · The Journal of Immunology · doi:10.4049/jimmunol.158.12.5632 · Cited by 61
Self-citation
The delta T cell receptor repertoire in human colon and peripheral blood is oligoclonal irrespective of V region usage.
Holtmeier, W., Chowers, Y., Lumeng, A., Morzycka-Wroblewska, E., & Kagnoff, M. F. (1995) · Journal of Clinical Investigation · doi:10.1172/jci118097 · Cited by 58
Self-citation
Microanatomic clonality of gamma delta T cells in human leishmaniasis lesions
Uyemura, K., Klotz, J. L., Pirmez, C., Ohmen, J. D., Wang, X. H., Ho, C. T., Hoffman, W. L., & Modlin, R. L. (1992) · The Journal of Immunology · doi:10.4049/jimmunol.148.4.1205 · Cited by 56
Clonal expansions of Vδ1+ and Vδ2+ cells increase with age and limit the repertoire of human γδ T cells
Giachino, C., Granzierou, L., Modenau, V., Maioccou, V., Lomateru, C., Fantiniu, F., Lanzavecchia, A., & Migonef, N. (1994) · European Journal of Immunology · doi:10.1002/eji.1830240830 · Cited by 54
T lymphocytes bearing the γδ T-cell receptor: a study in normal human skin and pathological skin conditions
Alaibac, M., Morris, J. F., Yu, R., & Chu, A. C. (1992) · British Journal of Dermatology · doi:10.1111/j.1365-2133.1992.tb14840.x · Cited by 49
Peripheral selection of V delta 1+ cells with restricted T cell receptor delta gene junctional repertoire in the peripheral blood of healthy donors.
Beldjord, K., Beldjord, C., Macintyre, E. A., Even, P., & Sigaux, F. (1993) · The Journal of Experimental Medicine · doi:10.1084/jem.178.1.121 · Cited by 44
Molecular and cellular biology of dendritic epidermal T cells
Boismenu, R. (1996) · Seminars in Immunology · doi:10.1006/smim.1996.0043 · Cited by 38
Unique selection determinant in polyclonal V delta 2-J delta 1 junctional regions of human peripheral gamma delta T lymphocytes.
Breit, T. M., Wolvers-Tettero, I. L. M., & van Dongen, J. J. M. (1994) · The Journal of Immunology · doi:10.4049/jimmunol.152.6.2860 · Cited by 36
T-Cell receptor αβ and γδ T cells in rat and human skin — are they equivalent?
Elbe, A. (1996) · Seminars in Immunology · doi:10.1006/smim.1996.0045 · Cited by 33
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Alaibac, M., Daga, A., Harms, G., Morris, J. F., Yu, R., Zwingerberger, K., & Chu, A. C. (1993) · Experimental Dermatology · doi:10.1111/j.1600-0625.1993.tb00017.x · Cited by 23
Comparative T cell receptor repertoire selection by antigen after adoptive transfer: A glimpse at an antigen-specific preimmune repertoire
Attuil, V., Bücher, P., Rossi, M., Mutin, M., & Maryanski, J. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.15.8473 · Cited by 16
Oligoclonality of Vδ1 and Vδ2 Cells in Human Peripheral Blood Mononuclear Cells: TCR Selection Is Not Altered by Stimulation with Gram-Negative Bacteria
Shen, J., Andrews, D. M., Pandolfi, F., Boyle, L. A., Kersten, C. M., Blatman, R. N., & Kurnick, J. T. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.160.6.3048 · Cited by 16
Identification and Characterization of Novel Dermal Thy-1 Antigen-Bearing Dendritic Cells in Murine Skin
Tamaki, K., Yasaka, N., Chang, C., OHTAKE, N., Saitoh, A., Nakamura, K., & Furue, M. (1996) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12344049 · Cited by 14
γδ T-cell receptor-positive cells in human skin. I. Incidence and V-region gene expression in granulomatous skin lesions
Fujita, M., Miyachi, Y., Nakata, K., & Imamura, S. (1993) · Journal of the American Academy of Dermatology · doi:10.1016/0190-9622(93)70007-g · Cited by 13
Analysis of the TCRBV Repertoire of T Cells in Normal, Human Skin: Evidence for a Restricted Diversity
Menssen, A., Vollmer, S., Trommler, P., Sander, C. A., & Prinz, J. C. (2000) · Journal of Investigative Dermatology · doi:10.1046/j.1523-1747.2000.00982.x · Cited by 11
γδ T-cells in human cutaneous immunology
Alaibac, M., Morris, J. F., & Chu, A. C. (1997) · International Journal of Clinical & Laboratory Research · doi:10.1007/bf02912452 · Cited by 9
High incidence of a T nucleotide at the second position of codon 97 in Vδ2-(D)-Jδ1 junctional sequences of human normal skin γδ T-cells
Alaibac, M., & Chu, A. C. (1996) · Molecular Immunology · doi:10.1016/s0161-5890(96)00067-3 · Cited by 3

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Authors: Holtmeier, W., Pfänder, M., Hennemann, A., Caspary, W. F., Zollner, T. M., & Kaufmann, R.
Affiliations: Goethe University Frankfurt (DE)
Corresponding author: Holtmeier, Wolfgang
ORCID: Zollner, Thomas M.
Venue: Journal of Investigative Dermatology
Publisher: Elsevier BV
Published: 2001-02
Volume: 116
Issue: 2
Pages: 275-280
ISSN: 0022-202X (ISSN-L)
DOI: 10.1046/j.1523-1747.2001.01250.x
OpenAlex: W2045500037
Cited by: 101
References: 44
Citations by year: 2026: 1 · 2025: 4 · 2024: 6 · 2023: 7 · 2022: 3 · 2021: 3 · 2020: 8 · 2019: 1 · 2018: 8 · 2017: 3 · 2016: 2 · 2015: 3 · 2014: 7 · 2013: 5 · 2012: 2
FWCI: 1.41
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 5417 d)
Locations: Journal of Investigative Dermatology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
Keywords: γδ T cells, T cell receptor delta chain, skin, peripheral blood, clonal expansion, T-cell receptor gene rearrangement, skin immune system, intestinal mucosa
MeSH terms: Clone Cells, Humans, Skin, Receptors, Antigen, T-Cell, gamma-delta, Gene Expression Profiling, Complementarity Determining Regions

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The TCR δ Repertoire in Normal Human Skin is Restricted and Distinct from the TCR δ Repertoire in the Peripheral Blood

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