The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Detection of Endotoxin in the Blood of Patients with Sepsis Due to Gram-Negative Bacteria
Abstract
A test for endotoxin was performed on the blood of 98 patients in whom septicemia due to gram-negative bacteria was suspected. The method was derived from a previous observation that endotoxin causes gelation of lysates of Limulus polyphemus amebocytes in vitro. Seventeen per cent of the patients studied had endotoxemia, as defined by this test. There was a good correlation between positive tests for endotoxin and bacteremia due to gram-negative organisms. Patients with pneumococcal pneumonia and bacteremia due to gram-positive organisms had negative tests. Endotoxin or endotoxin-like material was detected in the blood in concentrations from approximately 0.005 to 0.0005 μg per milliliter. Our data indicate that the limulus test is a sensitive, reliable and practical method for the detection of endotoxemia.
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Citations
Cites
Detection of endotoxin in human blood and demonstration of an inhibitor.
Levin, J., Tomasulo, P. A., & Oser, R. S. (1970) · PubMed · Cited by 428
Self-citationComparative Pyrogenic Reactivity of Rabbit and Man to Bacterial Endotoxin
Greisman, S. E., & Hornick, R. B. (1969) · Experimental Biology and Medicine · doi:10.3181/00379727-131-34059 · Cited by 149
The Limulus Coagulation Test for Endotoxin. A Comparison with Other Assay Methods
Rojas-Corona, R. R., Skarnes, R. C., Tamakuma, S., et al. (1969) · Experimental Biology and Medicine · doi:10.3181/00379727-132-34268 · Cited by 90
The Endotoxins of Gram-Negative Bacteria and Host Resistance
Rosen, F. S. (1961) · New England Journal of Medicine · doi:10.1056/nejm196105042641807 · Cited by 50
The Use of a Bioassay for Endotoxin in Clinical Infections
McGill, M. W., Porter, P. J., & KASS, E. H. (1970) · The Journal of Infectious Diseases · doi:10.1093/infdis/121.2.103 · Cited by 32
Endotoxin-like Activity of Serum from Patients with Severe Localized Infections
Porter, P. J., Spievack, A. R., & Kass, E. H. (1964) · New England Journal of Medicine · doi:10.1056/nejm196408272710905 · Cited by 31
Cited by
Guthrie, L. A., McPhail, L. C., Henson, P. M., & Johnston, R. B. (1984) · The Journal of experimental medicine · doi:10.1084/jem.160.6.1656 · Cited by 616
Smedly, L. A., Tonnesen, M. G., Sandhaus, R. A., Haslett, C., Guthrie, L. A., Johnston, R. B., Henson, P. M., & Worthen, G. S. (1986) · Journal of Clinical Investigation · doi:10.1172/jci112426 · Cited by 567
Endotoxin and Bacteria in Portal Blood
Jacob, A. I., Goldberg, P. K., Bloom, N., Degenshein, G. A., & Kozinn, P. J. (1977) · Gastroenterology · doi:10.1016/s0016-5085(77)80025-5 · Cited by 393
Endotoxemia: Methods of detection and clinical correlates
Hurley, J. C. (1995) · Clinical Microbiology Reviews · doi:10.1128/cmr.8.2.268 · Cited by 318
Endotoxemia—menace, marker, or mistake?
Munford, R. S. (2016) · Journal of Leukocyte Biology · doi:10.1189/jlb.3ru0316-151r · Cited by 219
“in 1970 [52, 56], the assay has since been widely used to detect endotoxin in fluids and is now the”
Method
Metadata
- Authors
- Levin, J., Poore, T. E., Zauber, N. P., & Oser, R. S.
- Venue
- New England Journal of Medicine
- Publisher
- Massachusetts Medical Society
- Published
- 1970-12-10
- Volume
- 283
- Issue
- 24
- Pages
- 1313-1316
- ISSN
- 0028-4793 (ISSN-L)
- 1533-4406
- OpenAlex
- W2069676891
- Cited by
- 317
- References
- 6
- FWCI
- 19.09
- Type
- journal article
- Language
- en
- Open access
- closed
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- Levin, J., Poore, T. E., Zauber, N. P., & Oser, R. S. (1970). Detection of Endotoxin in the Blood of Patients with Sepsis Due to Gram-Negative Bacteria. New England Journal of Medicine. https://doi.org/10.1056/nejm197012102832404
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
