The DOI matches its Crossref record; 28 of the 30 references it cites were found in a registry or an index.
Nitric Oxide as a Mediator of Relaxation of the Corpus Cavernosum in Response to Nonadrenergic, Noncholinergic Neurotransmission
Open accessAbstract
BACKGROUND: Nitric oxide has been identified as an endothelium-derived relaxing factor in blood vessels. We tried to determine whether it is involved in the relaxation of the corpus cavernosum that allows penile erection. The relaxation of this smooth muscle is known to occur in response to stimulation by nonadrenergic, noncholinergic neurons. METHODS: We studied strips of corpus cavernosum tissue obtained from 21 men in whom penile prostheses were inserted because of impotence. The mounted smooth-muscle specimens were pretreated with guanethidine and atropine and submaximally contracted with phenylephrine. We then studied the smooth-muscle relaxant responses to stimulation by an electrical field and to nitric oxide. RESULTS: Electrical-field stimulation caused a marked, transient, frequency-dependent relaxation of the corpus cavernosum that was inhibited in the presence of N-nitro-L-arginine and N-amino-L-arginine, which selectively inhibit the biosynthesis of nitric oxide from L-arginine. The addition of excess L-arginine, but not D-arginine, largely reversed these inhibitory effects. The specific liberation of nitric oxide (by S-nitroso-N-acetylpenicillamine) caused rapid, complete, and concentration-dependent relaxation of the corpus cavernosum. The relaxation caused by either electrical stimulation or nitric oxide was enhanced by a selective inhibitor of cyclic guanosine monophosphate (GMP) phosphodiesterase (M&B 22,948). Relaxation was inhibited by methylene blue, which inhibits cyclic GMP synthesis. CONCLUSIONS: Our findings support the hypothesis that nitric oxide is involved in the nonadrenergic, noncholinergic neurotransmission that leads to the smooth-muscle relaxation in the corpus cavernosum that permits penile erection. Defects in this pathway may cause some forms of impotence.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“NO synthesized by NOS in the endothelium diffuses into the vessel wall where it activates sGC in vascular smooth muscle; this leads to a rise in cyclic GMP (cGMP) and elicits relaxation of the vessel [96-102].”
From Regulation of obesity and insulin resistance by nitric oxide (2014) · cites this paper as [99]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Furchgott, R. F., & Zawadzki, J. V. (1980) · Nature · doi:10.1038/288373a0 · Cited by 12,064
Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor
Palmer, R. M. J., Ferrige, A. G., & Moncada, S. (1987) · Nature · doi:10.1038/327524a0 · Cited by 10,704
Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide.
Ignarro, L. J., Buga, G. M., Wood, K. S., Byrns, R. E., & Chaudhuri, G. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.24.9265 · Cited by 5,074
Vascular endothelial cells synthesize nitric oxide from L-arginine
Palmer, R. M. J., Ashton, D., & Moncada, S. (1988) · Nature · doi:10.1038/333664a0 · Cited by 4,786
Ignarro, L. J., Lippton, H., Edwards, J. C., Baricos, W. H., Hyman, A. L., Kadowitz, P. J., & Gruetter, C. A. (1981) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)32738-2 · Cited by 1,633
Ignarro, L. J. (1989) · Circulation Research · doi:10.1161/01.res.65.1.1 · Cited by 964
de Tejada, I. S., Goldstein, I., Azadzoi, K. M., Krane, R. J., & Cohen, R. A. (1989) · New England Journal of Medicine · doi:10.1056/nejm198904203201601 · Cited by 944
Ignarro, L. J., Bush, M. A., Buga, G. M., Wood, K. S., Fukuto, J. M., & Rajfer, J. (1990) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(90)92168-y · Cited by 832
The Pharmacological and Physiological Role of Cyclic GMP in Vascular Smooth Muscle Relaxation
Ignarro, L. J., & Kadowitz, P. J. (1985) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pa.25.040185.001131 · Cited by 679
Signal transduction mechanisms involving nitric oxide
Ignarro, L. J. (1991) · Biochemical Pharmacology · doi:10.1016/0006-2952(91)90618-f · Cited by 524
Research works printed but not linked: 3
- Barnes CD, Eltherington LG. Drug dosage in laboratory animals: a handbook. Berkeley: University of California Press, 1965:261.
- Dixon WJ, ed. BMDP statistical software manual. Vol. 2. Berkeley: University of California Press, 1990.
- Gruetter, 1979, J Cyclic Nucleotide Res, 5, 211
Cited by
Sorted by citations
Nitric Oxide and Peroxynitrite in Health and Disease
Pacher, P., Beckman, J. S., & Liaudet, L. (2007) · Physiological Reviews · doi:10.1152/physrev.00029.2006 · Cited by 6,390
Sexual Dysfunction in the United States: Prevalence and Predictors
Laumann, E. O., Paik, A., & Rosen, R. C. (1999) · JAMA · doi:10.1001/jama.281.6.537 · Cited by 5,119
Nitric oxide synthases: regulation and function
Forstermann, U., & Sessa, W. C. (2012) · European Heart Journal · doi:10.1093/eurheartj/ehr304 · Cited by 4,409
Nitric oxide as a secretory product of mammalian cells
Nathan, C. (1992) · The FASEB Journal · doi:10.1096/fasebj.6.12.1381691 · Cited by 4,350
Oral Sildenafil in the Treatment of Erectile Dysfunction
Goldstein, I., Lue, T. F., Padma-Nathan, H., Rosen, R. C., Steers, W. D., & Wicker, P. A. (1998) · New England Journal of Medicine · doi:10.1056/nejm199805143382001 · Cited by 2,120
Metadata
- Authors
- Rajfer, J., Aronson, W. J., Bush, P. A., Dorey, F. J., & Ignarro, L. J.
- Affiliations
- University of California, Los Angeles (US)
- ORCID
- Aronson, William J.
- Venue
- New England Journal of Medicine
- Publisher
- Massachusetts Medical Society
- Published
- 1992-01-09
- Volume
- 326
- Issue
- 2
- Pages
- 90-94
- ISSN
- 0028-4793 (ISSN-L)
- 1533-4406
- OpenAlex
- W2056147576
- Cited by
- 1,006
- References
- 30
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 5 · 2023: 11 · 2022: 12 · 2021: 11 · 2020: 13 · 2019: 14 · 2018: 15 · 2017: 17 · 2016: 17 · 2015: 17 · 2014: 26 · 2013: 22 · 2012: 30
- FWCI
- 50.38
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- New England Journal of Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01hl040922-11, 5r01hl035014-12)
- National Heart, Lung, and Blood Institute (HL-35014, HL 40922)
- Topics
- Health Sciences › Medicine › Psychiatry and Mental health › Sexual function and dysfunction studies
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Urology › Urinary Bladder and Prostate Research
- Keywords
- nitric oxide, corpus cavernosum, penile erection, non-adrenergic non-cholinergic neurotransmission, smooth muscle relaxation, erectile dysfunction, cyclic GMP, L-arginine, methylene blue, penile prosthesis, electrical field stimulation
- MeSH terms
- Adult, Aged, Arginine, Electric Stimulation, Cyclic GMP, Humans, Erectile Dysfunction, Male, Methylene Blue, Middle Aged, Muscle Relaxation, Muscle, Smooth, Synaptic Transmission, Nitric Oxide, Organ Culture Techniques, Penile Erection, Penis, Purinones, Nitroarginine
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
