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Rapid and Sensitive Spectrophotometry. III. A Double Beam Apparatus
Abstract
An apparatus for accurately timing the sequence of spectroscopic events in turbid suspensions of the cytochrome oxidase system has been developed. This apparatus measures simultaneously small optical density changes occurring at two wavelengths in the visible region with a small spectral interval in spite of the large scattering of light by the heart muscle suspensions. The cross talk between the two channels corresponds to an optical density change of less than 5×10−4. Transients arising from the modulation and demodulation processes cause no difficulty. The error of optical measurements caused by the noise level in the photocurrent is 2×10−4 for a rise time (10–90 percent) of 1 sec. Some typical experimental results are included.
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Citations
Cited by
Jöbsis, F. F. (1977) · Science · doi:10.1126/science.929199 · Cited by 3,899
The Assay of Catalases and Peroxidases
Maehly, A. C. (1954) · Methods of Biochemical Analysis · doi:10.1002/9780470110171.ch14 · Cited by 3,303
[2] Citrate synthase from rat liver
Shepherd, D., & Garland, P. B. (1969) · Methods in Enzymology · doi:10.1016/0076-6879(69)13006-2 · Cited by 434
Time-resolved spectroscopy of hemoglobin and myoglobin in resting and ischemic muscle
Chance, B., Nioka, S., Kent, J., McCully, K., Fountain, M., Greenfeld, R., & Holtom, G. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90076-0 · Cited by 329
Self-citation
Metadata
- Authors
- Chance, B.
- Venue
- Review of Scientific Instruments
- Publisher
- AIP Publishing
- Published
- 1951-08-01
- Volume
- 22
- Issue
- 8
- Pages
- 634-638
- ISSN
- 0034-6748 (ISSN-L)
- 1089-7623
- OpenAlex
- W2014758513
- Cited by
- 296
- References
- 0
- FWCI
- 6.53
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
