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Molecular recognition at self-assembled monolayers: Optimization of surface functionalization
Abstract
Sulfur-based molecules containing biotin and hydroxyl groups have been used to create a wide variety of self-assembled monolayers on gold surfaces. Surface plasmon resonance has been used to study in situ the binding of streptavidin to these monolayers from solution. The self-assembled monolayers allow a high degree of control over the surface properties. The choice of an appropriate biotin-containing molecule, with a spacer segment, and the dilution of this molecule within the monolayer by hydroxythiols, allows optimization of the binding properties of the monolayer—nonspecific interactions between streptavidin and the surface are below detection limits, and specific binding between the streptavidin and biotin groups can be maximized.
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Citations
Cited by
Optical Amplification of Ligand-Receptor Binding Using Liquid Crystals
Gupta, V. K., Skaife, J. J., Dubrovsky, T. B., & Abbott, N. L. (1998) · Science · doi:10.1126/science.279.5359.2077 · Cited by 476
Luk, Y.-Y., Kato, M., & Mrksich, M. (2000) · Langmuir · doi:10.1021/la0004653 · Cited by 414
Metadata
- Authors
- Spinke, J., Liley, M., Schmitt, F.-J., Guder, H.-J., Angermaier, L., & Knoll, W.
- Venue
- The Journal of Chemical Physics
- Publisher
- AIP Publishing
- Published
- 1993-11-01
- Volume
- 99
- Issue
- 9
- Pages
- 7012-7019
- ISSN
- 0021-9606 (ISSN-L)
- 1089-7690
- OpenAlex
- W2014362134
- Cited by
- 313
- References
- 0
- FWCI
- 7.90
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Molecular recognition at self-assembled monolayers: Optimization of surface functionalization. https://app.cochat.ai/papers/10.1063/1.465447
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- Spinke, J., Liley, M., Schmitt, F.-J., Guder, H.-J., Angermaier, L., & Knoll, W. (1993). Molecular recognition at self-assembled monolayers: Optimization of surface functionalization. The Journal of Chemical Physics. https://doi.org/10.1063/1.465447
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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