Optical Amplification of Ligand-Receptor Binding Using Liquid Crystals
Abstract
Liquid crystals (LCs) were used to amplify and transduce receptor-mediated binding of proteins at surfaces into optical outputs. Spontaneously organized surfaces were designed so that protein molecules, upon binding to ligands hosted on these surfaces, triggered changes in the orientations of 1- to 20-micrometer-thick films of supported LCs, thus corresponding to a reorientation of ∼10 5 to 10 6 mesogens per protein. Binding-induced changes in the intensity of light transmitted through the LC were easily seen with the naked eye and could be further amplified by using surfaces designed so that protein-ligand recognition causes twisted nematic LCs to untwist. This approach to the detection of ligand-receptor binding does not require labeling of the analyte, does not require the use of electroanalytical apparatus, provides a spatial resolution of micrometers, and is sufficiently simple that it may find use in biochemical assays and imaging of spatially resolved chemical libraries.
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Technological advances in high-throughput screening
Fernandes, P. B. (1998) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(98)80089-6 · Cited by 170 · see citing works
- Authors
- Gupta, V. K., Skaife, J. J., Dubrovsky, T. B., & Abbott, N. L.
- Affiliations
- University of California, Davis (US)
- Corresponding author
- Gupta, Vinay K.
- Dubrovsky, Timothy B.
- Abbott, Nicholas L.
- ORCID
- Abbott, Nicholas L.
- Venue
- Science
- Published
- 1998-03-27
- Volume
- 279
- Issue
- 5359
- Pages
- 2077-2080
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1971689738
- Cited by
- 476
- References
- 0
- Citations by year
- 2026: 10 · 2025: 20 · 2024: 11 · 2023: 19 · 2022: 22 · 2021: 26 · 2020: 16 · 2019: 18 · 2018: 20 · 2017: 12 · 2016: 20 · 2015: 18 · 2014: 23 · 2013: 14 · 2012: 17
- FWCI
- 8.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Liquid Crystal Research Advancements
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Keywords
- liquid crystals, optical amplification, ligand-receptor binding, protein-ligand recognition, twisted nematic liquid crystal, label-free detection, biochemical tests
- MeSH terms
- Antigen-Antibody Reactions, Avidin, Biotin, Crystallization, Gold, Immunoglobulin G, Ligands, Protein Binding, Anisotropy, Biotinylation, Optics and Photonics
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