Recombinant allergens promote expression of CD203c on basophils in sensitized individuals
Open accessAbstract
Background Traditionally, the diagnosis of type I allergies is based on clinical data, skin test results, and laboratory test results with allergen extracts. During the past few years, several attempts have been made to refine diagnostic assays in clinical allergy by introducing recombinant allergens and novel markers of IgE-dependent cell activation.Objectives We have identified the ectoenzyme CD203c as a novel basophil antigen that is upregulated on IgE receptor cross-linkage. In this study we applied CD203c and a panel of recombinant allergens to establish a novel basophil test that allows for a reliable quantification of IgE-dependent responses at the effector cell level.Methods Patients allergic to birch (Bet v 1, n = 15; Bet v 2, n = 8) and grass (Phl p 1, n = 15; Phl p 2, n = 10; Phl p 5, n = 14) pollen allergens, as well as 10 nonallergic donors, were examined. Basophils were exposed to various concentrations of recombinant allergens for 15 minutes and then examined for expression of CD203c by means of flow cytometry. CD203c upregulation was correlated with the increase in CD63.Results Exposure to recombinant allergens resulted in a dose-dependent increase in expression of CD203c on peripheral blood basophils in sensitized individuals, whereas no increase was seen in healthy control subjects. The effects of the recombinant allergens on CD203c expression were also time dependent. There was a good correlation between allergen-induced upregulation of CD203c and upregulation of CD63 (R = 0.76).Conclusion Flow cytometric quantitation of CD203c on blood basophils exposed to recombinant allergens is a useful approach to determine the allergic state in sensitized individuals and represents a basis for a sensitive novel allergy test.
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CD63 and CD203c cell surface levels are reliable and established parameters of basophil and MC activation.
“Reliable and established parameters of basophil- and MC activation include analysis of cell surface levels of CD63 and/or CD203c (51-55).”
From Proposed Diagnostic Algorithm for Patients with Suspected Mast Cell Activation Syndrome (2019) · cites this paper as [52]
The Discussion (page unavailable) describes CD63 as a marker used in basophil activation tests and CD203c as a sensitive but novel marker of allergen-induced basophil activation. The paper supports their use for basophils, but does not establish them as parameters of mast-cell (MC) activation or describe CD203c as already established. (LLM, conf=0.87)
Experimental — accuracy not yet measuredCD63 and CD203c increase on the surface of FcεR cross-linked MC and basophils.
“Both proteins (antigens) increase on the surface of FcεR cross-linked MC and basophils (51-55).”
From Proposed Diagnostic Algorithm for Patients with Suspected Mast Cell Activation Syndrome (2019) · cites this paper as [52]
The section on CD203c and CD63 upregulation reports that allergen stimulation increased both markers on basophils, and the Discussion (p. 107) describes rapid CD203c upregulation after FcεRI cross-linking. The paper does not establish the claimed increase on mast cells (MC), so it supports the basophil portion only. (LLM, conf=0.88)
Experimental — accuracy not yet measured
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Self-citationBasophil Activation Test in Allergy: Time for an Update?
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Self-citationAssays for measuring in vitro basophil activation induced by recombinant allergens
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Self-citationDissection of the IgE and T-cell recognition of the major group 5 grass pollen allergen Phl p 5
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Altered IgE epitope presentation: A model for hypoallergenic activity revealed for Bet v 1 trimer
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Characterization of recombinant cat albumin
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Self-citationBeyond Skin Testing: State of the Art and New Horizons in Food Allergy Diagnostic Testing
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Self-citationExpression and regulation of Siglec-6 (CD327) on human mast cells and basophils
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Self-citationEpitope Mapping of Rhi o 1 and Generation of a Hypoallergenic Variant
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RetractedExtracorporeal IgE Immunoadsorption in Allergic Asthma: Safety and Efficacy
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Self-citationMast cells derived from human induced pluripotent stem cells are useful for allergen tests
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Self-citation
- Authors
- Hauswirth, A. W., Natter, S., Ghannadan, M., Majlesi, Y., Schernthaner, G.-H., Sperr, W. R., Bühring, H.-J., Valenta, R., & Valent, P.
- Affiliations
- University of Vienna (AT)
- FWF Austrian Science Fund (AT)
- Venue
- Journal of Allergy and Clinical Immunology
- Publisher
- Elsevier BV
- Published
- 2002-07
- Volume
- 110
- Issue
- 1
- Pages
- 102-109
- ISSN
- 0091-6749 (ISSN-L)
- OpenAlex
- W2075788391
- Cited by
- 169
- References
- 34
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 3 · 2022: 3 · 2021: 5 · 2020: 1 · 2019: 4 · 2018: 4 · 2017: 7 · 2016: 12 · 2015: 13 · 2014: 7 · 2013: 9 · 2012: 12
- FWCI
- 4.00
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Allergy and Clinical Immunology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Keywords
- CD203c, basophils, recombinant allergens, CD63, birch pollen allergen, grass pollen allergens, Bet v 1, Bet v 2, Phl p 1, Phl p 5, allergy diagnosis
- MeSH terms
- Allergens, Basophils, Female, Flow Cytometry, Poaceae, Histamine Release, Humans, Hypersensitivity, Immediate, Immunoglobulin E, Male, Phosphoric Diester Hydrolases, Plant Proteins, Platelet Membrane Glycoproteins, Pyrophosphatases, Recombinant Proteins, Antigens, CD, Up-Regulation, Betula, Tetraspanin 30
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