Cited by 170Allergic Rhinitis and SensitizationMast cells and histamineAsthma and respiratory diseases

Recombinant allergens promote expression of CD203c on basophils in sensitized individuals

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Hauswirth, Alexander W., Natter, Susanne, Ghannadan, Minoo, Majlesi, Yasamin, Schernthaner, Gerit-Holger, Sperr, Wolfgang R., Bühring, Hans-Jörg, Valenta, Rudolf +1 more·2002·Journal of Allergy and Clinical Immunology

Abstract

Background Traditionally, the diagnosis of type I allergies is based on clinical data, skin test results, and laboratory test results with allergen extracts. During the past few years, several attempts have been made to refine diagnostic assays in clinical allergy by introducing recombinant allergens and novel markers of IgE-dependent cell activation.Objectives We have identified the ectoenzyme CD203c as a novel basophil antigen that is upregulated on IgE receptor cross-linkage. In this study we applied CD203c and a panel of recombinant allergens to establish a novel basophil test that allows for a reliable quantification of IgE-dependent responses at the effector cell level.Methods Patients allergic to birch (Bet v 1, n = 15; Bet v 2, n = 8) and grass (Phl p 1, n = 15; Phl p 2, n = 10; Phl p 5, n = 14) pollen allergens, as well as 10 nonallergic donors, were examined. Basophils were exposed to various concentrations of recombinant allergens for 15 minutes and then examined for expression of CD203c by means of flow cytometry. CD203c upregulation was correlated with the increase in CD63.Results Exposure to recombinant allergens resulted in a dose-dependent increase in expression of CD203c on peripheral blood basophils in sensitized individuals, whereas no increase was seen in healthy control subjects. The effects of the recombinant allergens on CD203c expression were also time dependent. There was a good correlation between allergen-induced upregulation of CD203c and upregulation of CD63 (R = 0.76).Conclusion Flow cytometric quantitation of CD203c on blood basophils exposed to recombinant allergens is a useful approach to determine the allergic state in sensitized individuals and represents a basis for a sensitive novel allergy test.

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Crossref · 10.1067/mai.2002.125257

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  • CD63 and CD203c cell surface levels are reliable and established parameters of basophil and MC activation.

    “Reliable and established parameters of basophil- and MC activation include analysis of cell surface levels of CD63 and/or CD203c (51-55).”

    From Proposed Diagnostic Algorithm for Patients with Suspected Mast Cell Activation Syndrome (2019) · cites this paper as [52]

    The Discussion (page unavailable) describes CD63 as a marker used in basophil activation tests and CD203c as a sensitive but novel marker of allergen-induced basophil activation. The paper supports their use for basophils, but does not establish them as parameters of mast-cell (MC) activation or describe CD203c as already established. (LLM, conf=0.87)

    Experimental — accuracy not yet measured
  • CD63 and CD203c increase on the surface of FcεR cross-linked MC and basophils.

    “Both proteins (antigens) increase on the surface of FcεR cross-linked MC and basophils (51-55).”

    From Proposed Diagnostic Algorithm for Patients with Suspected Mast Cell Activation Syndrome (2019) · cites this paper as [52]

    The section on CD203c and CD63 upregulation reports that allergen stimulation increased both markers on basophils, and the Discussion (p. 107) describes rapid CD203c upregulation after FcεRI cross-linking. The paper does not establish the claimed increase on mast cells (MC), so it supports the basophil portion only. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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1 of the 34 references couldn’t be matched to a known paper.

Research works printed but not linked: 2

  1. [4]4. Valenta R, Vrtala S, Laffer S, Spitzauer S, Kraft D. Recombinant aller- gens. Allergy 1998;53:552-61.
  2. [28]28. Modderman PW. Cluster report CD63. In: Knapp W, et al, editors. Leukocyte typing IV. Oxford, United Kingdom: Oxford University Press; 1989. p. 1042

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Authors
Hauswirth, A. W., Natter, S., Ghannadan, M., Majlesi, Y., Schernthaner, G.-H., Sperr, W. R., Bühring, H.-J., Valenta, R., & Valent, P.
Affiliations
  • University of Vienna (AT)
  • FWF Austrian Science Fund (AT)
Venue
Journal of Allergy and Clinical Immunology
Publisher
Elsevier BV
Published
2002-07
Volume
110
Issue
1
Pages
102-109
ISSN
0091-6749 (ISSN-L)
OpenAlex
W2075788391
Cited by
169
References
34
Citations by year
2026: 1 · 2025: 2 · 2023: 3 · 2022: 3 · 2021: 5 · 2020: 1 · 2019: 4 · 2018: 4 · 2017: 7 · 2016: 12 · 2015: 13 · 2014: 7 · 2013: 9 · 2012: 12
FWCI
4.00
Type
journal article
Language
en
Open access
Locations
  • Journal of Allergy and Clinical Immunology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
  • Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
  • Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
Keywords
CD203c, basophils, recombinant allergens, CD63, birch pollen allergen, grass pollen allergens, Bet v 1, Bet v 2, Phl p 1, Phl p 5, allergy diagnosis
MeSH terms
Allergens, Basophils, Female, Flow Cytometry, Poaceae, Histamine Release, Humans, Hypersensitivity, Immediate, Immunoglobulin E, Male, Phosphoric Diester Hydrolases, Plant Proteins, Platelet Membrane Glycoproteins, Pyrophosphatases, Recombinant Proteins, Antigens, CD, Up-Regulation, Betula, Tetraspanin 30

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