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Recombinant allergens promote expression of CD203c on basophils in sensitized individuals
Open accessAbstract
Background Traditionally, the diagnosis of type I allergies is based on clinical data, skin test results, and laboratory test results with allergen extracts. During the past few years, several attempts have been made to refine diagnostic assays in clinical allergy by introducing recombinant allergens and novel markers of IgE-dependent cell activation.Objectives We have identified the ectoenzyme CD203c as a novel basophil antigen that is upregulated on IgE receptor cross-linkage. In this study we applied CD203c and a panel of recombinant allergens to establish a novel basophil test that allows for a reliable quantification of IgE-dependent responses at the effector cell level.Methods Patients allergic to birch (Bet v 1, n = 15; Bet v 2, n = 8) and grass (Phl p 1, n = 15; Phl p 2, n = 10; Phl p 5, n = 14) pollen allergens, as well as 10 nonallergic donors, were examined. Basophils were exposed to various concentrations of recombinant allergens for 15 minutes and then examined for expression of CD203c by means of flow cytometry. CD203c upregulation was correlated with the increase in CD63.Results Exposure to recombinant allergens resulted in a dose-dependent increase in expression of CD203c on peripheral blood basophils in sensitized individuals, whereas no increase was seen in healthy control subjects. The effects of the recombinant allergens on CD203c expression were also time dependent. There was a good correlation between allergen-induced upregulation of CD203c and upregulation of CD63 (R = 0.76).Conclusion Flow cytometric quantitation of CD203c on blood basophils exposed to recombinant allergens is a useful approach to determine the allergic state in sensitized individuals and represents a basis for a sensitive novel allergy test.
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“Reliable and established parameters of basophil- and MC activation include analysis of cell surface levels of CD63 and/or CD203c (51-55).”
From Proposed Diagnostic Algorithm for Patients with Suspected Mast Cell Activation Syndrome (2019) · cites this paper as [52]
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“Both proteins (antigens) increase on the surface of FcεR cross-linked MC and basophils (51-55).”
From Proposed Diagnostic Algorithm for Patients with Suspected Mast Cell Activation Syndrome (2019) · cites this paper as [52]
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Metadata
- Authors
- Hauswirth, A. W., Natter, S., Ghannadan, M., Majlesi, Y., Schernthaner, G.-H., Sperr, W. R., Bühring, H.-J., Valenta, R., & Valent, P.
- Affiliations
- University of Vienna (AT)
- FWF Austrian Science Fund (AT)
- Venue
- Journal of Allergy and Clinical Immunology
- Publisher
- Elsevier BV
- Published
- 2002-07
- Volume
- 110
- Issue
- 1
- Pages
- 102-109
- ISSN
- 0091-6749 (ISSN-L)
- OpenAlex
- W2075788391
- Cited by
- 169
- References
- 34
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 3 · 2022: 3 · 2021: 5 · 2020: 1 · 2019: 4 · 2018: 4 · 2017: 7 · 2016: 12 · 2015: 13 · 2014: 7 · 2013: 9 · 2012: 12
- FWCI
- 4.00
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Allergy and Clinical Immunology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Asthma and respiratory diseases
- Keywords
- CD203c, basophils, recombinant allergens, CD63, birch pollen allergen, grass pollen allergens, Bet v 1, Bet v 2, Phl p 1, Phl p 5, allergy diagnosis
- MeSH terms
- Allergens, Basophils, Female, Flow Cytometry, Poaceae, Histamine Release, Humans, Hypersensitivity, Immediate, Immunoglobulin E, Male, Phosphoric Diester Hydrolases, Plant Proteins, Platelet Membrane Glycoproteins, Pyrophosphatases, Recombinant Proteins, Antigens, CD, Up-Regulation, Betula, Tetraspanin 30
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