The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
A Ca 2+ /calmodulin-dependent protein kinase required for symbiotic nodule development: Gene identification by transcript-based cloning
Open accessAbstract
In the establishment of the legume-rhizobial symbiosis, bacterial lipochitooligosaccharide signaling molecules termed Nod factors activate the formation of a novel root organ, the nodule. Nod factors elicit several responses in plant root hair cells, including oscillations in cytoplasmic calcium levels (termed calcium spiking) and alterations in root hair growth. A number of plant mutants with defects in the Nod factor signaling pathway have been identified. One such Medicago truncatula mutant, dmi3 , exhibits calcium spiking and root hair swelling in response to Nod factor, but fails to initiate symbiotic gene expression or cell divisions for nodule formation. On the basis of these data, it is thought that the dmi3 mutant perceives Nod factor but fails to transduce the signal downstream of calcium spiking. Additionally, the dmi3 mutant is defective in the symbiosis with mycorrhizal fungi, indicating the importance of the encoded protein in multiple symbioses. We report the identification of the DMI3 gene, using a gene cloning method based on transcript abundance. We show that transcript-based cloning is a valid approach for cloning genes in barley, indicating the value of this technology in crop plants. DMI3 encodes a calcium/calmodulin-dependent protein kinase. Mutants in pea sym9 have phenotypes similar to dmi3 and have alterations in this gene. The DMI3 class of proteins is well conserved among plants that interact with mycorrhizal fungi, but it is less conserved in Arabidopsis thaliana , which does not participate in the mycorrhizal symbiosis.
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Cites
Calcium's Role in Mechanotransduction during Muscle Development
Benavides Damm, T., & Egli, M. (2014) · Cellular Physiology and Biochemistry · doi:10.1159/000356667 · Cited by 14,502
Model-based analysis of oligonucleotide arrays: Expression index computation and outlier detection.
Li, C., & Wong, W. H. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.98.1.31 · Cited by 3,133
Differential activation of transcription factors induced by Ca2+ response amplitude and duration
Dolmetsch, R. E., Lewis, R. S., Goodnow, C. C., & Healy, J. I. (1997) · Nature · doi:10.1038/386855a0 · Cited by 1,752
Differential activation of transcription factors induced by Ca2+ response amplitude and duration
Dolmetsch, R. E., Lewis, R. S., Goodnow, C. C., & Healy, J. I. (1997) · Nature · doi:10.1038/40912 · Cited by 1,521
Sensitivity of CaM Kinase II to the Frequency of Ca 2+ Oscillations
De Koninck, P., & Schulman, H. (1998) · Science · doi:10.1126/science.279.5348.227 · Cited by 1,269
Plant recognition of symbiotic bacteria requires two LysM receptor-like kinases
Radutoiu, S., Madsen, L. H., Madsen, E. B., Felle, H. H., Umehara, Y., Grønlund, M., Sato, S., Nakamura, Y., Tabata, S., Sandal, N., et al. (2003) · Nature · doi:10.1038/nature02039 · Cited by 1,221
Lerouge, P., Roche, P., Faucher, C., Maillet, F., Truchet, G., Promé, J. C., & Dénarié, J. (1990) · Nature · doi:10.1038/344781a0 · Cited by 1,166
A receptor kinase gene of the LysM type is involved in legumeperception of rhizobial signals
Madsen, E. B., Madsen, L. H., Radutoiu, S., Olbryt, M., Rakwalska, M., Szczyglowski, K., Sato, S., Kaneko, T., Tabata, S., Sandal, N., et al. (2003) · Nature · doi:10.1038/nature02045 · Cited by 1,018
Li, C., & Hung Wong, W. (2001) · Genome Biology · doi:10.1186/gb-2001-2-8-research0032 · Cited by 976
Cell-permeant caged InsP3 ester shows that Ca2+ spike frequency can optimize gene expression
Li, W.-H., Llopis, J., Whitney, M., Zlokarnik, G., & Tsien, R. Y. (1998) · Nature · doi:10.1038/31965 · Cited by 844
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Metadata
- Authors
- Mitra, R. M., Gleason, C. A., Edwards, A., Hadfield, J., Downie, J. A., Oldroyd, G. E. D., & Long, S. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-03
- Volume
- 101
- Issue
- 13
- Pages
- 4701-4705
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1870053901
- Cited by
- 494
- References
- 33
- FWCI
- 44.24
- Type
- journal article
- Language
- en
- Open access
- green
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- Mitra, R. M., Gleason, C. A., Edwards, A., Hadfield, J., Downie, J. A., Oldroyd, G. E. D., & Long, S. R. (2004). A Ca 2+ /calmodulin-dependent protein kinase required for symbiotic nodule development: Gene identification by transcript-based cloning. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0400595101
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