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Cited by 440

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Labeling of fusion proteins with synthetic fluorophores in live cells

Open access
Keppler, Antje, Pick, Horst, Arrivoli, Claudio, Vogel, Horst, Johnsson, Kai·2004·Proceedings of the National Academy of Sciences

Abstract

A general approach for the sequential labeling of fusion proteins of O 6 -alkylguanine-DNA alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT fusion proteins with different localizations in the cell can be labeled specifically with different fluorophores, and the fluorescence labeling can be used for applications such as multicolor analysis of dynamic processes and fluorescence resonance energy transfer measurements. The facile access to a variety of different AGT substrates as well as the specificity of the labeling reaction should make the approach an important tool to study protein function in live cells.

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Crossref · 10.1073/pnas.0401923101

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Metadata

Authors
Keppler, A., Pick, H., Arrivoli, C., Vogel, H., & Johnsson, K.
Venue
Proceedings of the National Academy of Sciences
Published
2004-06-28
Volume
101
Issue
27
Pages
9955-9959
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W1970270735
Cited by
440
References
1
FWCI
11.49
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Labeling of fusion proteins with synthetic fluorophores in live cells. https://app.cochat.ai/papers/10.1073/pnas.0401923101
The paper
Keppler, A., Pick, H., Arrivoli, C., Vogel, H., & Johnsson, K. (2004). Labeling of fusion proteins with synthetic fluorophores in live cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0401923101

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.