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Characterization of purified intraembryonic hematopoietic stem cells as a tool to define their site of origin
Open accessAbstract
Little is known about hematopoietic stem cell (HSC) development from mesoderm. To gain more information on the intraembryonic HSC site of origin, we purified multipotent hematopoietic progenitors from the aorta–gonads–mesonephros (AGM) of mice. This population, expressing c-Kit, AA4.1, CD31, and CD41, but not Flk1, and mainly negative for CD45, proved capable of long-term reconstitution in sublethally irradiated Rag2γc -/- recipients. We assigned the expression of GATA-2, GATA-3, and lmo2 to AGM-HSC, whereas erythromyeloid progenitors express only GATA-2. This unique combination of surface markers and transcription factors could be allocated in the AGM to the intraaortic clusters and the subaortic patches underlying aortic endothelial cells. Taken together, those data indicate that embryonic HSCs ( i ) differ from their fetal liver and adult counterpart by the low expression of CD45, ( ii ) do not colocalize with aortic endothelial cells as previously thought, and ( iii ) are localized, at 10.5 days postcoïtum, in the splanchnic mesoderm underlying aortic endothelial cells, within GATA-3 + CD31 + cell clusters.
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Citations
Cited by
Hematopoiesis: An Evolving Paradigm for Stem Cell Biology
Orkin, S. H., & Zon, L. I. (2008) · Cell · doi:10.1016/j.cell.2008.01.025 · Cited by 2,570
Tissue-resident macrophages originate from yolk-sac-derived erythro-myeloid progenitors
Gomez Perdiguero, E., Klapproth, K., Schulz, C., Busch, K., Azzoni, E., Crozet, L., Garner, H., Trouillet, C., de Bruijn, M. F., Geissmann, F., et al. (2015) · Nature · doi:10.1038/nature13989 · Cited by 2,339
Haematopoietic stem cells derive directly from aortic endothelium during development
Bertrand, J. Y., Chi, N. C., Santoso, B., Teng, S., Stainier, D. Y. R., & Traver, D. (2010) · Nature · doi:10.1038/nature08738 · Cited by 1,025
Self-citationSpecification of tissue-resident macrophages during organogenesis
Mass, E., Ballesteros, I., Farlik, M., Halbritter, F., Günther, P., Crozet, L., Jacome-Galarza, C. E., Händler, K., Klughammer, J., Kobayashi, Y., et al. (2016) · Science · doi:10.1126/science.aaf4238 · Cited by 891
Of lineage and legacy: the development of mammalian hematopoietic stem cells
Dzierzak, E., & Speck, N. A. (2008) · Nature Immunology · doi:10.1038/ni1560 · Cited by 707
Metadata
- Authors
- Bertrand, J. Y., Giroux, S., Golub, R., Klaine, M., Jalil, A., Boucontet, L., Godin, I., & Cumano, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-12-27
- Volume
- 102
- Issue
- 1
- Pages
- 134-139
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2047695455
- Cited by
- 283
- References
- 0
- FWCI
- 9.81
- Type
- journal article
- Language
- en
- Open access
- green
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- Bertrand, J. Y., Giroux, S., Golub, R., Klaine, M., Jalil, A., Boucontet, L., Godin, I., & Cumano, A. (2005). Characterization of purified intraembryonic hematopoietic stem cells as a tool to define their site of origin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0402270102
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