The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A molecular link between SR protein dephosphorylation and mRNA export
Abstract
In metazoans, multiple RNA-binding proteins, including the shuttling serine/arginine-rich (SR)-splicing factors, function as adapters for mRNA nuclear export by interacting with the export receptor TAP/nuclear export factor 1 (NXF1). Yet, it is unclear how interactions between adapters and TAP are regulated. Here, we demonstrate that the SR proteins 9G8 and ASF/SF2 exhibit higher affinity for TAP/NXF1 when hypophosphorylated. 9G8 is recruited to the pre-mRNA in a hyperphosphorylated form but becomes hypophosphorylated during splicing both in vivo and in vitro . TAP preferentially binds spliced mRNA–protein complexes compared with pre-mRNA–protein complexes. Thus, the phosphorylation state of the SR protein adapters may underlie the selectivity of TAP-mediated export of spliced mRNA.
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“SRSF3 associates with spliceosomal components prior to release by dephosphorylation, at which point the mRNP interacts with the export receptor (Huang et al., 2004).”
From YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs (2017) · cites this paper as Huang et al., 2004
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“Indeed, SRSF3 is known to interact with both the splicing and the export machinery, and is known to do so in a phosphorylation-dependent manner, with hypo-phosphorylated protein interacting with the export receptor (Huang et al., 2004).”
From YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs (2017) · cites this paper as Huang et al., 2004
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Citations
Cites
A Conserved mRNA Export Machinery Coupled to pre-mRNA Splicing
Reed, R., & Hurt, E. (2002) · Cell · doi:10.1016/s0092-8674(02)00627-x · Cited by 400
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Cited by
Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches
Chen, M., & Manley, J. L. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2777 · Cited by 1,284
YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs
Roundtree, I. A., Luo, G.-Z., Zhang, Z., Wang, X., Zhou, T., Cui, Y., Sha, J., Huang, X., Guerrero, L., Xie, P., et al. (2017) · eLife · doi:10.7554/elife.31311 · Cited by 1,256
Sun, D., Novotny, M., Bulek, K., Liu, C., Li, X., & Hamilton, T. (2011) · Nature Immunology · doi:10.1038/ni.2081 · Cited by 232
Metadata
- Authors
- Huang, Y., Yario, T. A., & Steitz, J. A.
- Affiliations
- Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520; and Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2004-06-21
- Volume
- 101
- Issue
- 26
- Pages
- 9666-9670
- ISSN
- 0027-8424
- 1091-6490
- Cited by
- 243
- References
- 1
- Language
- en
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- Huang, Y., Yario, T. A., & Steitz, J. A. (2004). A molecular link between SR protein dephosphorylation and mRNA export. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0403533101
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