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Multiple-stress analysis for isolation of Drosophila longevity genes
Open accessAbstract
Long-lived organisms tend to be more resistant to various forms of environmental stress. An example is the Drosophila longevity mutant,methuselah, which has enhanced resistance to heat, oxidants, and starvation. To identify genes regulated by these three stresses, we made a cDNA library for each by subtraction of “unstressed” from “stressed” cDNA and used DNA hybridization to identify genes that are regulated by all three. This screen indeed identified 13 genes, some already known to be involved in longevity, plus candidate genes. Two of these,hsp26 and hsp27, were chosen to test for their effects on lifespan by generating transgenic lines and by using the upstream activating sequence/GAL4 system. Overexpression of either hsp26 or hsp27 extended the mean lifespan by 30%, and the flies also displayed increased stress resistance. The results demonstrate that multiple-stress screening can be used to identify new longevity genes.
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Cited by
The plasticity of aging: insights from long-lived mutants
Kenyon, C. (2005) · Cell · doi:10.1016/j.cell.2005.02.002 · Cited by 1,338
Functional senescence in Drosophila melanogaster
Grotewiel, M. S., Martin, I., Bhandari, P., & Cook-Wiens, E. (2005) · Ageing Research Reviews · doi:10.1016/j.arr.2005.04.001 · Cited by 323
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Evolutionary biology of starvation resistance: what we have learned from Drosophila
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Polycomb Repressive Complex 2 and Trithorax modulate Drosophila longevity and stress resistance
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Metadata
- Authors
- Wang, H.-D., Kazemi-Esfarjani, P., & Benzer, S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-08-12
- Volume
- 101
- Issue
- 34
- Pages
- 12610-12615
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2001641785
- Cited by
- 196
- References
- 5
- FWCI
- 4.25
- Type
- journal article
- Language
- en
- Open access
- green
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