The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography
Open accessAbstract
We used cryo-electron tomography in conjunction with single-particle averaging techniques to study the structures of frozen-hydrated envelope glycoprotein (Env) complexes on intact Moloney murine leukemia retrovirus particles. Cryo-electron tomography allows 3D imaging of viruses in toto at a resolution sufficient to locate individual macromolecules, and local averaging of abundant complexes substantially improves the resolution. The averaging of repetitive features in electron tomograms is hampered by a low signal-to-noise ratio and anisotropic resolution, which results from the “missing-wedge” effect. We developed an iterative 3D averaging algorithm that compensates for this effect and used it to determine the trimeric structure of Env to a resolution of 2.7 nm, at which individual domains can be resolved. Strikingly, the 3D reconstruction is shaped like a tripod in which the trimer penetrates the membrane at three distinct locations ≈4.5 nm apart from one another. The Env reconstruction allows tentative docking of the x-ray crystal structure of the receptor-binding domain. This study thus provides 3D structural information regarding the prefusion conformation of an intact unstained retrovirus surface protein.
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Cites
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Self-citationAtomic structure of the ectodomain from HIV-1 gp41
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Cited by
Neuman, B. W., Adair, B. D., Yoshioka, C., Quispe, J. D., Orca, G., Kuhn, P., Milligan, R. A., Yeager, M., & Buchmeier, M. J. (2006) · Journal of Virology · doi:10.1128/jvi.00645-06 · Cited by 438
Structure and assembly of immature HIV
Briggs, J. A. G., Riches, J. D., Glass, B., Bartonova, V., Zanetti, G., & Kräusslich, H.-G. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0903535106 · Cited by 368
Structure of the Prefusion Form of the Vesicular Stomatitis Virus Glycoprotein G
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Integrated Structural Analysis of the Human Nuclear Pore Complex Scaffold
Bui, K. H., von Appen, A., DiGuilio, A. L., Ori, A., Sparks, L., Mackmull, M.-T., Bock, T., Hagen, W., Andrés-Pons, A., Glavy, J. S., et al. (2013) · Cell · doi:10.1016/j.cell.2013.10.055 · Cited by 357
A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0
Zivanov, J., Otón, J., Ke, Z., von Kügelgen, A., Pyle, E., Qu, K., Morado, D., Castaño-Díez, D., Zanetti, G., Bharat, T. A., et al. (2022) · eLife · doi:10.7554/elife.83724 · Cited by 339
Metadata
- Authors
- Förster, F., Medalia, O., Zauberman, N., Baumeister, W., & Fass, D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2005-03-17
- Volume
- 102
- Issue
- 13
- Pages
- 4729-4734
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2046542456
- Cited by
- 302
- References
- 34
- FWCI
- 16.41
- Type
- journal article
- Language
- en
- Open access
- green
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- Förster, F., Medalia, O., Zauberman, N., Baumeister, W., & Fass, D. (2005). Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0409178102
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