The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Hysteresis in a synthetic mammalian gene network
Open accessAbstract
Bistable and hysteretic switches, enabling cells to adopt multiple internal expression states in response to a single external input signal, have a pivotal impact on biological systems, ranging from cell-fate decisions to cell-cycle control. We have designed a synthetic hysteretic mammalian transcription network. A positive feedback loop, consisting of a transgene and transactivator (TA) cotranscribed by TA's cognate promoter, is repressed by constitutive expression of a macrolide-dependent transcriptional silencer, whose activity is modulated by the macrolide antibiotic erythromycin. The antibiotic concentration, at which a quasi-discontinuous switch of transgene expression occurs, depends on the history of the synthetic transcription circuitry. If the network components are imbalanced, a graded rather than a quasi-discontinuous signal integration takes place. These findings are consistent with a mathematical model. Synthetic gene networks, which are able to emulate natural gene expression behavior, may foster progress in future gene therapy and tissue engineering initiatives.
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Cited by
Network motifs: theory and experimental approaches
Alon, U. (2007) · Nature Reviews Genetics · doi:10.1038/nrg2102 · Cited by 3,279
Nature, Nurture, or Chance: Stochastic Gene Expression and Its Consequences
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Synthetic biology: applications come of age
Khalil, A. S., & Collins, J. J. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2775 · Cited by 1,373
Genetic circuit design automation
Nielsen, A. A. K., Der, B. S., Shin, J., Vaidyanathan, P., Paralanov, V., Strychalski, E. A., Ross, D., Densmore, D., & Voigt, C. A. (2016) · Science · doi:10.1126/science.aac7341 · Cited by 1,020
Phenotypic Consequences of Promoter-Mediated Transcriptional Noise
Blake, W. J., Balázsi, G., Kohanski, M. A., Isaacs, F. J., Murphy, K. F., Kuang, Y., Cantor, C. R., Walt, D. R., & Collins, J. J. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.11.003 · Cited by 629
Metadata
- Authors
- Kramer, B. P., & Fussenegger, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2005-06-22
- Volume
- 102
- Issue
- 27
- Pages
- 9517-9522
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2145641640
- Cited by
- 240
- References
- 7
- FWCI
- 6.91
- Type
- journal article
- Language
- en
- Open access
- green
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