Cited by 128Ubiquitin and proteasome pathwaysCancer-related Molecular PathwaysMicrotubule and mitosis dynamics

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β-TrCP recognizes a previously undescribed nonphosphorylated destruction motif in Cdc25A and Cdc25B phosphatases

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Kanemori, Yoshinori, Uto, Katsuhiro, Sagata, Noriyuki·2005·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.0501873102PMC PMC1083676

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Abstract

β-TrCP, the F-box protein of the SCF β-TrCP ubiquitin ligase (SCF, Skp1/Cul1/F-box protein), recognizes the doubly phosphorylated DSG motif (DpSGΦXpS) in various SCF β-TrCP target proteins. The Cdc25A phosphatase, a key cell-cycle regulator in vertebrate cells, undergoes a rapid ubiquitin-dependent degradation in response to genotoxic stress. β-TrCP binds to the DSG motif of human Cdc25A in a manner dependent on Chk1 and other unknown kinases. However, Xenopus Cdc25A does not have a DSG motif at the corresponding site of human Cdc25A. Here, we report that both Xenopus Cdc25A and human Cdc25A have a previously undescribed nonphosphorylated DDG motif (DDGΦXD) for recognition by β-TrCP. When analyzed by using Xenopus eggs, the binding of β-TrCP to the DDG motif is essential for the Chk1-induced ubiquitination and degradation of Xenopus Cdc25A and also plays a role in the degradation of human Cdc25A. The DDG motif also exists in human Cdc25B phosphatase (another key cell-cycle regulator), binds β-TrCP strongly, and is essential for the ubiquitination and degradation of the (labile) phosphatase in normal conditions. We provide strong evidence that, in both Cdc25A and Cdc25B, the binding (efficiency) of β-TrCP to the DDG motif is regulated by nearby residues, while ubiquitination is regulated by other events in addition to the β-TrCP binding. Finally, our additional data suggest that β-TrCP may recognize nonphosphorylated DDG-like motifs in many other proteins, including X11L (a putative suppressor of β-amyloid production) and hnRNP-U (a pseudosubstrate of SCF β-TrCP ).

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Authors: Kanemori, Y., Uto, K., & Sagata, N.
Affiliations: Kyushu University (JP)
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2005-04-21
Volume: 102
Issue: 18
Pages: 6279-6284
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.0501873102
PMC: PMC1083676
OpenAlex: W2110323618
Cited by: 128
References: 42
Citations by year: 2026: 4 · 2025: 4 · 2024: 2 · 2023: 6 · 2022: 4 · 2021: 2 · 2020: 7 · 2019: 4 · 2018: 4 · 2017: 5 · 2016: 3 · 2015: 4 · 2014: 9 · 2013: 2 · 2012: 6
FWCI: 4.27
Type: journal article
Language: en
Open access: green
dx.doi.org
Licence: other-oa
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion, other-oa)
Europe PMC (PubMed Central) (publishedVersion)
PubMed (publishedVersion)
Funders: Japan Science and Technology Agency
Core Research for Evolutional Science and Technology
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords: β-TrCP, SCF ubiquitin ligase, CDC25A, CDC25B, ubiquitination, protein degradation, Chk1, genotoxic stress, cell cycle regulation, HNRNPU
MeSH terms: Checkpoint Kinase 1, Animals, Glutathione Transferase, Humans, Ovum, Protein Binding, Protein Kinases, Ubiquitins, Xenopus, Immunoblotting, Mutagenesis, DNA, Complementary, Cell Cycle Proteins, cdc25 Phosphatases, Amino Acid Motifs, Xenopus Proteins, Ubiquitin-Protein Ligases, beta-Transducin Repeat-Containing Proteins

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β-TrCP recognizes a previously undescribed nonphosphorylated destruction motif in Cdc25A and Cdc25B phosphatases

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