Iroquois transcription factors recognize a unique motif to mediate transcriptional repression in vivo

Bilioni, Aphrodite; Craig, Gavin; Hill, Caroline; McNeill, Helen

doi:10.1073/pnas.0502480102

Cited by 71Genomics and Chromatin DynamicsDevelopmental Biology and Gene RegulationProtein Structure and Dynamics

Iroquois transcription factors recognize a unique motif to mediate transcriptional repression in vivo

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Bilioni, Aphrodite, Craig, Gavin, Hill, Caroline, McNeill, Helen·2005·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.0502480102PMC PMC1239941

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Abstract

Iroquois transcription factors regulate diverse aspects of developmental patterning in all metazoans. Despite their widespread importance, the direct targets of the Iroquois are poorly understood. Here, we use in vitro site selection to define the DNA-binding preference of the Drosophila Iroquois Mirror. We use electrophoretic mobility shift assays to determine the critical nucleotides for Mirror binding and to show that this site is recognized by other Drosophila Iroquois transcription factors. This site also is recognized by vertebrate Iroquois transcription factors. Transgenic analysis demonstrates that Drosophila Iroquois proteins recognize this site in vivo to mediate transcriptional repression. We further show that Iroquois transcription factors form homodimers and heterodimers, suggesting that combinatorial binding may contribute to gene regulation by this family.

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Similar interactions between homologues have been reported for many other transcription factor families, such as Ebf and Irx.
“Similar interactions between homologues have been reported for many other transcription factor families, such as Ebf98 and Irx99.”
From A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain (2023) · cites this paper as [99]

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Authors: Bilioni, A., Craig, G., Hill, C., & McNeill, H.
Affiliations: Cancer Research UK (GB)
ORCID: Hill, Caroline
McNeill, Helen
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2005-10-03
Volume: 102
Issue: 41
Pages: 14671-14676
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.0502480102
PMC: PMC1239941
OpenAlex: W1979675055
Cited by: 71
References: 26
Citations by year: 2026: 2 · 2025: 1 · 2024: 1 · 2023: 7 · 2022: 7 · 2021: 2 · 2020: 3 · 2018: 4 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 6 · 2013: 5 · 2012: 6
FWCI: 1.26
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords: transcriptional repression, DNA-binding motifs, Drosophila, homodimerization, heterodimerization, combinatorial gene regulation, developmental patterning
MeSH terms: Amino Acid Sequence, Animals, Base Sequence, DNA, Drosophila, Eye Proteins, Gene Expression Regulation, Luciferases, Molecular Sequence Data, Oligonucleotides, Protein Binding, Transcription Factors, Sequence Alignment, N-Acetylglucosaminyltransferases, Homeodomain Proteins, Dimerization, Electrophoretic Mobility Shift Assay, Drosophila Proteins

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Iroquois transcription factors recognize a unique motif to mediate transcriptional repression in vivo

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