CoChat
Sign Up
Cited by 71Biochemical and Structural CharacterizationStreptococcal Infections and TreatmentsEnzyme Structure and Function

Structure of the streptococcal cell wall C5a peptidase.

Open access
Brown, C. Kent, Gu, Zu-Yi, Matsuka, Yury V., Purushothaman, Sai S., Winter, Laurie A., Cleary, P. Patrick, Olmsted, Stephen B., Ohlendorf, Douglas H. +1 more·2005·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0504954102PMC PMC1317908
Open this paper in CoChat — free account

Abstract

The structure of a cell surface enzyme from a gram-positive pathogen has been determined to 2-A resolution. Gram-positive pathogens have a thick cell wall to which proteins and carbohydrate are covalently attached. Streptococcal C5a peptidase (SCP), is a highly specific protease and adhesin/invasin. Structural analysis of a 949-residue fragment of the [D130A,S512A] mutant of SCP from group B Streptococcus (S. agalactiae, SCPB) revealed SCPB is composed of five distinct domains. The N-terminal subtilisin-like protease domain has a 134-residue protease-associated domain inserted into a loop between two beta-strands. This domain also contains one of two Arg-Gly-Asp (RGD) sequences found in SCPB. At the C terminus are three fibronectin type III (Fn) domains. The second RGD sequence is located between Fn1 and Fn2. Our analysis suggests that SCP binding to integrins by the RGD motifs may stabilize conformational changes required for substrate binding.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1073/pnas.0504954102

Cites

39 of 39 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The structure determined by Brown et al. (2005) was obtained with a recombinant mutant protein expressed in E. coli with the asp and ser of catalytic triad replaced by alanines and without pro-peptide; this structure has the histidine of catalytic triad separated by 20 Å from active position.

    “The structure determined by Brown et al. (2005) was obtained with a recombinant mutant protein expressed in Escherichia coli with the asp and ser of the catalytic triad replaced by alanines and without the pro-peptide. This structure has the histidine of the catalytic triad separated by 20 Å from the active position (Brown et al., 2005).”

    From Modeled Structure of the Cell Envelope Proteinase of Lactococcus lactis (2020) · cites this paper as Brown et al., 2005

    Split into 2 atoms — 0 confirmed, 2 partially supported, 0 disputed, 0 misattributed, 0 unverifiable. Expand the claim to see per-atom verdicts.

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 2

  1. Cleary, P. P., Matsuka, Y. V., Huynh, T., Lam, H. & Olmsted, S. B. (2004) Vaccine 22, 5332–5341.
  2. Dauter, Z., Betzel, C., Genov, N., Pipon, N. & Wilson, K. S. (1991) Acta Crystallogr. B 47, 707–730.1793542

Cited by

Sorted by citations

Related

Authors
Brown, C. K., Gu, Z.-Y., Matsuka, Y. V., Purushothaman, S. S., Winter, L. A., Cleary, P. P., Olmsted, S. B., Ohlendorf, D. H., & Earhart, C. A.
Affiliations
University of Minnesota (US)
ORCID
Brown, C. Kent
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2005-12-12
Volume
102
Issue
51
Pages
18391-18396
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0504954102
PMC
PMC1317908
OpenAlex
W2135032011
Semantic Scholar
e0eebf4a87e90b31d1371fadcc27abe2764177da
Cited by
70
References
39
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2023: 5 · 2022: 2 · 2021: 5 · 2020: 6 · 2019: 2 · 2018: 5 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 6 · 2013: 2 · 2012: 3
FWCI
0.82
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Minnesota
  • National Institutes of Health
  • National Institute on Aging (R01AG050607)
  • National Institute of Allergy and Infectious Diseases (R01AI020016, R01 AI50607, R01AI057585)
  • Brookhaven National Laboratory
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Streptococcal Infections and Treatments
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
Keywords
protein structure, RGD motif, integrin binding, fibronectin type III domain, adhesins, conformational change, substrate binding, Gram-positive bacterial cell wall
MeSH terms
Amino Acid Sequence, Cell Wall, Models, Molecular, Endopeptidases, Protein Binding, Streptococcus agalactiae, Protein Structure, Tertiary, Crystallography, X-Ray, Adhesins, Bacterial, Amino Acid Motifs, Protein Structure, Quaternary

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review