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Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis
Open accessAbstract
We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ, YgjV, YiaB, YigG, and YnfA. We estimate that our approach should make it possible to arrive at highly reliable topology models for roughly 10% of the ≈800 inner-membrane proteins thought to exist in E. coli .
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Citations
Cited by
Global Topology Analysis of the Escherichia coli Inner Membrane Proteome
Daley, D. O., Rapp, M., Granseth, E., Melén, K., Drew, D., & von Heijne, G. (2005) · Science · doi:10.1126/science.1109730 · Cited by 490
Self-citationOptimization of membrane protein overexpression and purification using GFP fusions
Drew, D., Lerch, M., Kunji, E., Slotboom, D.-J., & de Gier, J.-W. (2006) · Nature Methods · doi:10.1038/nmeth0406-303 · Cited by 339
Self-citationProteomics of Integral Membrane ProteinsTheory and Application
Speers, A. E., & Wu, C. C. (2007) · Chemical Reviews · doi:10.1021/cr068286z · Cited by 325
Metadata
- Authors
- Drew, D., Sjöstrand, D., Nilsson, J., Urbig, T., Chin, C.-N., de Gier, J.-W., & von Heijne, G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-02-26
- Volume
- 99
- Issue
- 5
- Pages
- 2690-2695
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2098623724
- Cited by
- 199
- References
- 0
- FWCI
- 6.50
- Type
- journal article
- Language
- en
- Open access
- green
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- Drew, D., Sjöstrand, D., Nilsson, J., Urbig, T., Chin, C.-N., de Gier, J.-W., & von Heijne, G. (2002). Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.052018199
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