The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana
Open accessAbstract
We investigated the potential of double-stranded RNA interference (RNAi) with gene activity in Arabidopsis thaliana . To construct transformation vectors that produce RNAs capable of duplex formation, gene-specific sequences in the sense and antisense orientations were linked and placed under the control of a strong viral promoter. When introduced into the genome of A. thaliana by Agrobacterium -mediated transformation, double-stranded RNA-expressing constructs corresponding to four genes, AGAMOUS ( AG ), CLAVATA3 , APETALA1 , and PERIANTHIA , caused specific and heritable genetic interference. The severity of phenotypes varied between transgenic lines. In situ hybridization revealed a correlation between a declining AG mRNA accumulation and increasingly severe phenotypes in AG ( RNAi ) mutants, suggesting that endogenous mRNA is the target of double-stranded RNA-mediated genetic interference. The ability to generate stably heritable RNAi and the resultant specific phenotypes allows us to selectively reduce gene function in A. thaliana .
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Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Early flower development in Arabidopsis.
Smyth, D. R., Bowman, J. L., & Meyerowitz, E. M. (1990) · The Plant Cell · doi:10.1105/tpc.2.8.755 · Cited by 2,155
Self-citation
Cited by
Seki, M., Narusaka, M., Ishida, J., Nanjo, T., Fujita, M., Oono, Y., Kamiya, A., Nakajima, M., Enju, A., Sakurai, T., et al. (2002) · The Plant Journal · doi:10.1046/j.1365-313x.2002.01359.x · Cited by 1,924
Ketting, R. F., Fischer, S. E. J., Bernstein, E., Sijen, T., Hannon, G. J., & Plasterk, R. H. A. (2001) · Genes & Development · doi:10.1101/gad.927801 · Cited by 1,852
RNA Interference: Biology, Mechanism, and Applications
Agrawal, N., Dasaradhi, P. V. N., Mohmmed, A., Malhotra, P., Bhatnagar, R. K., & Mukherjee, S. K. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.4.657-685.2003 · Cited by 1,305
Technical Advance: Tobacco rattle virus as a vector for analysis of gene function by silencing
Ratcliff, F., Martin‐Hernandez, A. M., & Baulcombe, D. C. (2001) · The Plant Journal · doi:10.1046/j.0960-7412.2000.00942.x · Cited by 960
Transcriptional silencing and promoter methylation triggered by double-stranded RNA
Mette, M. F., Aufsatz, W., van der Winden, J., Matzke, M. A., & Matzke, A. J. M. (2000) · The EMBO Journal · doi:10.1093/emboj/19.19.5194 · Cited by 869
Metadata
- Authors
- Chuang, C.-F., & Meyerowitz, E. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-04-18
- Volume
- 97
- Issue
- 9
- Pages
- 4985-4990
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2077489356
- Cited by
- 646
- References
- 2
- FWCI
- 98.61
- Type
- journal article
- Language
- en
- Open access
- green
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