Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans
Abstract
Double-stranded RNAs can suppress expression of homologous genes through an evolutionarily conserved process named RNA interference (RNAi) or post-transcriptional gene silencing (PTGS). One mechanism underlying silencing is degradation of target mRNAs by an RNP complex, which contains ∼22 nt of siRNAs as guides to substrate selection. A bidentate nuclease called Dicer has been implicated as the protein responsible for siRNA production. Here we characterize the Caenorhabditis elegans ortholog of Dicer ( K12H4.8 ; dcr-1 ) in vivo and in vitro. dcr-1 mutants show a defect in RNAi. Furthermore, a combination of phenotypic abnormalities and RNA analysis suggests a role for dcr-1 in a regulatory pathway comprised of small temporal RNA ( let-7 ) and its target (e.g., lin-41 ).
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The enzyme responsible for cleavage of miRNA hairpin precursors is Dicer.
“The enzyme responsible for this cleavage is Dicer (Hutvagner et al. 2001;Ketting et al. 2001).”
From A microRNA array reveals extensive regulation of microRNAs during brain development (2003) · cites this paper as Ketting et al. 2001
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- Authors
- Ketting, R. F., Fischer, S. E. J., Bernstein, E., Sijen, T., Hannon, G. J., & Plasterk, R. H. A.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2001-10-15
- Volume
- 15
- Issue
- 20
- Pages
- 2654-2659
- ISSN
- 0890-9369
- 1549-5477
- Cited by
- 1,527
- References
- 0
- Language
- en
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