The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Isolation of the Cdc45/Mcm2–7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase
Abstract
The protein Cdc45 plays a critical but poorly understood role in the initiation and elongation stages of eukaryotic DNA replication. To study Cdc45's function in DNA replication, we purified Cdc45 protein from Drosophila embryo extracts by a combination of traditional and immunoaffinity chromatography steps and found that the protein exists in a stable, high-molecular-weight complex with the Mcm2–7 hexamer and the GINS tetramer. The purified Cdc45/Mcm2–7/GINS complex is associated with an active ATP-dependent DNA helicase function. RNA interference knock-down experiments targeting the GINS and Cdc45 components establish that the proteins are required for the S phase transition in Drosophila cells. The data suggest that this complex forms the core helicase machinery for eukaryotic DNA replication.
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Cites
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S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex
Katou, Y., Kanoh, Y., Bando, M., Noguchi, H., Tanaka, H., Ashikari, T., Sugimoto, K., & Shirahige, K. (2003) · Nature · doi:10.1038/nature01900 · Cited by 648
Uninterrupted MCM2-7 Function Required for DNA Replication Fork Progression
Labib, K., Tercero, J. A., & Diffley, J. F. X. (2000) · Science · doi:10.1126/science.288.5471.1643 · Cited by 625
Eukaryotic MCM Proteins: Beyond Replication Initiation
Forsburg, S. L. (2004) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.68.1.109-131.2004 · Cited by 584
Self-Assembling Protein Microarrays
Ramachandran, N., Hainsworth, E., Bhullar, B., Eisenstein, S., Rosen, B., Lau, A. Y., Walter, J. C., & LaBaer, J. (2004) · Science · doi:10.1126/science.1097639 · Cited by 548
A DNA Helicase Activity Is Associated with an MCM4, -6, and -7 Protein Complex
Ishimi, Y. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.39.24508 · Cited by 538
Moir, D., Stewart, S. E., Osmond, B. C., & Botstein, D. (1982) · Genetics · doi:10.1093/genetics/100.4.547 · Cited by 373
GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast
Takayama, Y., Kamimura, Y., Okawa, M., Muramatsu, S., Sugino, A., & Araki, H. (2003) · Genes & Development · doi:10.1101/gad.1065903 · Cited by 372
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Cited by
Meier, F., Geyer, P. E., Virreira Winter, S., Cox, J., & Mann, M. (2018) · Nature Methods · doi:10.1038/s41592-018-0003-5 · Cited by 348
Mechanisms for initiating cellular DNA replication
Bleichert, F., Botchan, M. R., & Berger, J. M. (2017) · Science · doi:10.1126/science.aah6317 · Cited by 220
Self-citationMeechan, D. W., Tucker, E. S., Maynard, T. M., & LaMantia, A.-S. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0905696106 · Cited by 173
A global profile of replicative polymerase usage
Daigaku, Y., Keszthelyi, A., Müller, C. A., Miyabe, I., Brooks, T., Retkute, R., Hubank, M., Nieduszynski, C. A., & Carr, A. M. (2015) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2962 · Cited by 169
Mechanisms of replication fork protection: a safeguard for genome stability
Errico, A., & Costanzo, V. (2012) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409238.2012.655374 · Cited by 159
Metadata
- Authors
- Moyer, S. E., Lewis, P. W., & Botchan, M. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-07-05
- Volume
- 103
- Issue
- 27
- Pages
- 10236-10241
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2105413529
- Cited by
- 699
- References
- 42
- FWCI
- 14.73
- Type
- journal article
- Language
- en
- Open access
- closed
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- Moyer, S. E., Lewis, P. W., & Botchan, M. R. (2006). Isolation of the Cdc45/Mcm2–7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0602400103
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