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Uninterrupted MCM2-7 Function Required for DNA Replication Fork Progression
Abstract
Little is known about the DNA helicases required for the elongation phase of eukaryotic chromosome replication. Minichromosome maintenance (MCM) protein complexes have DNA helicase activity but have only been functionally implicated in initiating DNA replication. Using an improved method for constructing conditional degron mutants, we show that depletion of MCMs after initiation irreversibly blocks the progression of replication forks in Saccharomyces cerevisiae . Like the Escherichia coli dnaB and SV40 T antigen helicases, therefore, the MCM complex is loaded at origins before initiation and is essential for elongation. Restricting MCM loading to the G 1 phase ensures that initiation and elongation occur just once per cell cycle.
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Cited by
An auxin-based degron system for the rapid depletion of proteins in nonplant cells
Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T., & Kanemaki, M. (2009) · Nature Methods · doi:10.1038/nmeth.1401 · Cited by 1,663
Banaszynski, L. A., Chen, L.-C., Maynard-Smith, L. A., Ooi, A. G. L., & Wandless, T. J. (2006) · Cell · doi:10.1016/j.cell.2006.07.025 · Cited by 902
Maintaining genome stability at the replication fork
Branzei, D., & Foiani, M. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2852 · Cited by 786
Chimenti, C., Kajstura, J., Torella, D., Urbanek, K., Heleniak, H., Colussi, C., Di Meglio, F., Nadal-Ginard, B., Frustaci, A., Leri, A., et al. (2003) · Circulation Research · doi:10.1161/01.res.0000093985.76901.af · Cited by 386
Initiating DNA synthesis: from recruiting to activating the MCM complex
Lei, M., & Tye, B. K. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.8.1447 · Cited by 365
Metadata
- Authors
- Labib, K., Tercero, J. A., & Diffley, J. F. X.
- Venue
- Science
- Published
- 2000-06-02
- Volume
- 288
- Issue
- 5471
- Pages
- 1643-1647
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1968074226
- Cited by
- 625
- References
- 0
- FWCI
- 16.11
- Type
- journal article
- Language
- en
- Open access
- closed
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- Labib, K., Tercero, J. A., & Diffley, J. F. X. (2000). Uninterrupted MCM2-7 Function Required for DNA Replication Fork Progression. Science. https://doi.org/10.1126/science.288.5471.1643
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
