The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Convergence of isoprene and polyketide biosynthetic machinery: Isoprenyl- S -carrier proteins in the pksX pathway of Bacillus subtilis
Open accessAbstract
The pksX gene cluster from Bacillus subtilis is predicted to encode the biosynthesis of an as yet uncharacterized hybrid nonribosomal peptide/polyketide secondary metabolite. We used a combination of biochemical and mass spectrometric techniques to assign functional roles to the proteins AcpK, PksC, PksL, PksF, PksG, PksH, and PksI, and we conclude that they act to incorporate an acetate-derived β-methyl branch on an acetoacetyl- S -carrier protein and ultimately generate a Δ 2 -isoprenyl- S -carrier protein. This work highlights the power of mass spectrometry to elucidate the functions of orphan biosynthetic enzymes, and it details a mechanism by which single-carbon β-branches can be inserted into polyketide-like structures. This pathway represents a noncanonical route to the construction of prenyl units and serves as a prototype for the intersection of isoprenoid and polyketide biosynthetic manifolds in other natural product biosynthetic pathways.
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Cites
The complete genome sequence of the gram-positive bacterium Bacillus subtilis.
Kunst, F., Ogasawara, N., Moszer, I., Albertini, A. M., Alloni, G., Azevedo, V., Bertero, M. G., Bessières, P., Bolotin, A., Borchert, S., et al. (1997) · Nature · doi:10.1038/36786 · Cited by 3,679
Quadri, L. E. N., Weinreb, P. H., Lei, M., Nakano, M. M., Zuber, P., & Walsh, C. T. (1998) · Biochemistry · doi:10.1021/bi9719861 · Cited by 693
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Cited by
Metabolites from symbiotic bacteria
Piel, J. (2009) · Nat. Prod. Rep. · doi:10.1039/b703499g · Cited by 577
Udwary, D. W., Zeigler, L., Asolkar, R. N., Singan, V., Lapidus, A., Fenical, W., Jensen, P. R., & Moore, B. S. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0700962104 · Cited by 536
Natural Products Version 2.0: Connecting Genes to Molecules
Walsh, C. T., & Fischbach, M. A. (2010) · Journal of the American Chemical Society · doi:10.1021/ja909118a · Cited by 468
Self-citationBiosynthesis of polyketides by trans-AT polyketide synthases
Piel, J. (2010) · Natural Product Reports · doi:10.1039/b816430b · Cited by 304
Metadata
- Authors
- Calderone, C. T., Kowtoniuk, W. E., Kelleher, N. L., Walsh, C. T., & Dorrestein, P. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-06-13
- Volume
- 103
- Issue
- 24
- Pages
- 8977-8982
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2099302541
- Cited by
- 176
- References
- 2
- FWCI
- 10.57
- Type
- journal article
- Language
- en
- Open access
- green
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- Calderone, C. T., Kowtoniuk, W. E., Kelleher, N. L., Walsh, C. T., & Dorrestein, P. C. (2006). Convergence of isoprene and polyketide biosynthetic machinery: Isoprenyl- S -carrier proteins in the pksX pathway of Bacillus subtilis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0603148103
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