The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation
Open accessAbstract
DNA methylation is a stable epigenetic mark for transcriptional gene silencing in diverse organisms including plants and many animals. In contrast to the well characterized mechanism of DNA methylation by methyltransferases, the mechanisms and function of active DNA demethylation have been controversial. Genetic evidence suggested that the DNA glycosylase domain-containing protein ROS1 of Arabidopsis is a putative DNA demethylase, because loss-of-function ros1 mutations cause DNA hypermethylation and enhance transcriptional gene silencing. We report here the biochemical characterization of ROS1 and the effect of its overexpression on the DNA methylation of target genes. Our data suggest that the DNA glycosylase activity of ROS1 removes 5-methylcytosine from the DNA backbone and then its lyase activity cleaves the DNA backbone at the site of 5-methylcytosine removal by successive β- and δ-elimination reactions. Overexpression of ROS1 in transgenic plants led to a reduced level of cytosine methylation and increased expression of a target gene. These results demonstrate that ROS1 is a 5-methylcytosine DNA glycosylase/lyase important for active DNA demethylation in Arabidopsis .
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
DNA methylation patterns and epigenetic memory
Bird, A. (2002) · Genes & Development · doi:10.1101/gad.947102 · Cited by 7,124
DNA methylation and human disease
Robertson, K. D. (2005) · Nature Reviews Genetics · doi:10.1038/nrg1655 · Cited by 2,864
A Gateway Cloning Vector Set for High-Throughput Functional Analysis of Genes in Planta
Curtis, M. D., & Grossniklaus, U. (2003) · Plant Physiology · doi:10.1104/pp.103.027979 · Cited by 2,644
The epigenetic progenitor origin of human cancer
Feinberg, A. P., Ohlsson, R., & Henikoff, S. (2006) · Nature Reviews Genetics · doi:10.1038/nrg1748 · Cited by 1,853
Methylation-Induced Repression— Belts, Braces, and Chromatin
Bird, A. P., & Wolffe, A. P. (1999) · Cell · doi:10.1016/s0092-8674(00)81532-9 · Cited by 1,827
MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex
Ng, H.-H., Zhang, Y., Hendrich, B., Johnson, C. A., Turner, B. M., Erdjument-Bromage, H., Tempst, P., Reinberg, D., & Bird, A. (1999) · Nature Genetics · doi:10.1038/12659 · Cited by 936
Gardening the genome: DNA methylation in Arabidopsis thaliana
Chan, S. W.-L., Henderson, I. R., & Jacobsen, S. E. (2005) · Nature Reviews Genetics · doi:10.1038/nrg1601 · Cited by 891
RNAi-mediated pathways in the nucleus
Matzke, M. A., & Birchler, J. A. (2005) · Nature Reviews Genetics · doi:10.1038/nrg1500 · Cited by 844
Choi, Y., Gehring, M., Johnson, L., Hannon, M., Harada, J. J., Goldberg, R. B., Jacobsen, S. E., & Fischer, R. L. (2002) · Cell · doi:10.1016/s0092-8674(02)00807-3 · Cited by 796
Cited by
Epigenetic regulation of stress responses in plants
Chinnusamy, V., & Zhu, J.-K. (2009) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2008.12.006 · Cited by 1,162
Self-citationEpigenetic inheritance in plants
Henderson, I. R., & Jacobsen, S. E. (2007) · Nature · doi:10.1038/nature05917 · Cited by 835
Active DNA Demethylation Mediated by DNA Glycosylases
Zhu, J.-K. (2009) · Annual Review of Genetics · doi:10.1146/annurev-genet-102108-134205 · Cited by 745
Self-citationRegulation and function of DNA methylation in plants and animals
He, X.-J., Chen, T., & Zhu, J.-K. (2011) · Cell Research · doi:10.1038/cr.2011.23 · Cited by 555
Self-citationEpigenetic Regulation in Plants
Pikaard, C. S., & Mittelsten Scheid, O. (2014) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a019315 · Cited by 440
Metadata
- Authors
- Agius, F., Kapoor, A., & Zhu, J.-K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-08
- Volume
- 103
- Issue
- 31
- Pages
- 11796-11801
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2170828323
- Cited by
- 317
- References
- 36
- FWCI
- 7.49
- Type
- journal article
- Language
- en
- Open access
- green
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