The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
A genetically encoded fluorescent amino acid
Open accessAbstract
The ability to introduce fluorophores selectively into proteins provides a powerful tool to study protein structure, dynamics, localization, and biomolecular interactions both in vitro and in vivo . Here, we report a strategy for the selective and efficient biosynthetic incorporation of a low-molecular-weight fluorophore into proteins at defined sites. The fluorescent amino acid 2-amino-3-(5-(dimethylamino)naphthalene-1-sulfonamide)propanoic acid (dansylalanine) was genetically encoded in Saccharomyces cerevisiae by using an amber nonsense codon and corresponding orthogonal tRNA/aminoacyl-tRNA synthetase pair. This environmentally sensitive fluorophore was selectively introduced into human superoxide dismutase and used to monitor unfolding of the protein in the presence of guanidinium chloride. The strategy described here should be applicable to a number of different fluorophores in both prokaryotic and eukaryotic organisms, and it should facilitate both biochemical and cellular studies of protein structure and function.
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Self-citationHu, C.-D., & Kerppola, T. K. (2003) · Nature Biotechnology · doi:10.1038/nbt816 · Cited by 781
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Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality
Sletten, E. M., & Bertozzi, C. R. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200900942 · Cited by 3,165
Adding New Chemistries to the Genetic Code
Liu, C. C., & Schultz, P. G. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.052308.105824 · Cited by 1,725
Self-citationExpanding and Reprogramming the Genetic Code of Cells and Animals
Chin, J. W. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035737 · Cited by 476
Self-citationA chemical toolkit for proteins — an expanded genetic code
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Self-citationNext-generation synthetic gene networks
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Metadata
- Authors
- Summerer, D., Chen, S., Wu, N., Deiters, A., Chin, J. W., & Schultz, P. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-06-27
- Volume
- 103
- Issue
- 26
- Pages
- 9785-9789
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1978880815
- Cited by
- 273
- References
- 25
- FWCI
- 6.51
- Type
- journal article
- Language
- en
- Open access
- green
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- Summerer, D., Chen, S., Wu, N., Deiters, A., Chin, J. W., & Schultz, P. G. (2006). A genetically encoded fluorescent amino acid. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0603965103
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