An immunocompetent mouse model for the tolerance of human chronic hepatitis B virus infection
Open accessAbstract
An animal model for human hepatitis B virus (HBV) tolerance is needed to investigate the mechanisms. This model will also facilitate therapeutic strategies for the existing 350 million patients with chronic hepatitis B. We established a mouse model by hydrodynamic injection of an engineered, replication-competent HBV DNA into the tail veins of C57BL/6 mice. In 40% of the injected mice, HBV surface antigenemia persisted for > 6 months. Viral replication intermediates, transcripts, and proteins were detected in the liver tissues of the injected mice for up to 1 year. The tolerance toward HBV surface antigen in this model was shown to be due to an insufficient cellular immunity against hepatitis B core antigen, as was documented in humans. This animal model will accelerate further genetic and mechanistic studies of human chronic hepatitis B infection.
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The HBV hydrodynamics-mouse model is a well-established model of HBV persistence.
“The HBV hydrodynamics-mouse model is a well-established model of HBV persistence.28,29”
From The CRISPR/Cas9 system facilitates clearance of the intrahepatic HBV templates in vivo (2014) · cites this paper as [28]
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The CRISPR/Cas9 system facilitates clearance of the intrahepatic HBV templates in vivo
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- Authors
- Huang, L.-R., Wu, H.-L., Chen, P.-J., & Chen, D.-S.
- Affiliations
- National Taiwan University (TW)
- Hepatitis Research Center,
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2006-11-21
- Volume
- 103
- Issue
- 47
- Pages
- 17862-17867
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC1635544
- OpenAlex
- W1992349567
- Cited by
- 294
- References
- 38
- Citations by year
- 2026: 1 · 2025: 20 · 2024: 16 · 2023: 22 · 2022: 18 · 2021: 23 · 2020: 21 · 2019: 19 · 2018: 19 · 2017: 22 · 2016: 20 · 2015: 22 · 2014: 13 · 2013: 21 · 2012: 14
- FWCI
- 2.70
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Hepatitis B Virus Studies
- Health Sciences › Medicine › Hepatology › Hepatitis C virus research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- chronic hepatitis B, mouse model, hydrodynamic injection, hepatitis B surface antigen, HBcAg, cell-mediated immunity, viral replication, immunocompetent mice
- MeSH terms
- Animals, Antigens, Viral, Disease Models, Animal, Hepatitis B virus, Humans, Immunocompetence, Interferon-gamma, Liver, Male, Mice, Inbred BALB C, Mice, Inbred C57BL, Plasmids, Hepatitis B, Chronic, Mice
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