Cited by 294Hepatitis B Virus StudiesHepatitis C virus researchLiver physiology and pathology

An immunocompetent mouse model for the tolerance of human chronic hepatitis B virus infection

Open access
Huang, Li-Rung, Wu, Hui-Lin, Chen, Pei-Jer, Chen, Ding-Shinn·2006·Proceedings of the National Academy of Sciences

Abstract

An animal model for human hepatitis B virus (HBV) tolerance is needed to investigate the mechanisms. This model will also facilitate therapeutic strategies for the existing 350 million patients with chronic hepatitis B. We established a mouse model by hydrodynamic injection of an engineered, replication-competent HBV DNA into the tail veins of C57BL/6 mice. In 40% of the injected mice, HBV surface antigenemia persisted for > 6 months. Viral replication intermediates, transcripts, and proteins were detected in the liver tissues of the injected mice for up to 1 year. The tolerance toward HBV surface antigen in this model was shown to be due to an insufficient cellular immunity against hepatitis B core antigen, as was documented in humans. This animal model will accelerate further genetic and mechanistic studies of human chronic hepatitis B infection.

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Crossref · 10.1073/pnas.0608578103

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Authors
Huang, L.-R., Wu, H.-L., Chen, P.-J., & Chen, D.-S.
Affiliations
  • National Taiwan University (TW)
  • Hepatitis Research Center,
Venue
Proceedings of the National Academy of Sciences
Published
2006-11-21
Volume
103
Issue
47
Pages
17862-17867
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W1992349567
Cited by
294
References
38
Citations by year
2026: 1 · 2025: 20 · 2024: 16 · 2023: 22 · 2022: 18 · 2021: 23 · 2020: 21 · 2019: 19 · 2018: 19 · 2017: 22 · 2016: 20 · 2015: 22 · 2014: 13 · 2013: 21 · 2012: 14
FWCI
2.70
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Epidemiology › Hepatitis B Virus Studies
  • Health Sciences › Medicine › Hepatology › Hepatitis C virus research
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Keywords
chronic hepatitis B, mouse model, hydrodynamic injection, hepatitis B surface antigen, HBcAg, cell-mediated immunity, viral replication, immunocompetent mice
MeSH terms
Animals, Antigens, Viral, Disease Models, Animal, Hepatitis B virus, Humans, Immunocompetence, Interferon-gamma, Liver, Male, Mice, Inbred BALB C, Mice, Inbred C57BL, Plasmids, Hepatitis B, Chronic, Mice

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